PDF(Pubmed)
Abstract：
This report provides a detailed overview of the resurgence of DENV-3 in the state of Minas Gerais, Brazil, which is a concerning scenario in the context of dengue, a mosquito-borne viral disease. Historically, Brazil has grappled with dengue epidemics caused primarily by the DENV-1 and DENV-2 serotypes. However, in 2023, a significant shift in this pattern was observed as DENV-3 made a notable resurgence. This resurgence was characterized by the increase in DENV-3 cases within the country and the region of the Americas. Given the absence of sustained DENV-3 circulation in Brazil in previous years, this situation poses a significant risk, making the population highly susceptible to a potential novel epidemic. In November 2023, a 31-year-old male patient in Belo Horizonte exhibited symptoms of acute febrile syndrome. Multiplex RT-qPCR using the Kit Molecular ZC D-Tipagem confirmed DENV-3 infection, suggesting a likely autochthonous case, as the patient reported no travel history. To promptly assess this resurgence, we applied the nanopore sequencing technology. This allowed for the rapid characterization of the initial DENV-3 case isolated in Minas Gerais in 2023, representing a 13-year interval since the serotype\'s previous documented circulation in that state. This case report underscores the critical importance of proactive monitoring and the swift implementation of targeted control strategies to address the evolving dynamics of dengue, with a specific emphasis on the resurgence of DENV-3 in the state.
摘要：
本报告详细概述了DENV-3在米纳斯吉拉斯州的复兴，巴西,这是登革热背景下的一个令人担忧的场景，一种蚊媒病毒病.历史上,巴西已努力应对主要由DENV-1和DENV-2血清型引起的登革热流行。然而,在2023年,由于DENV-3显著复苏,观察到该模式发生了显著变化.这种复苏的特点是该国和美洲区域DENV-3病例增加。鉴于巴西前几年没有持续的DENV-3流通，这种情况带来了巨大的风险，使人口高度容易受到潜在的新流行病的影响。2023年11月，贝洛奥里藏特的一名31岁男性患者表现出急性发热综合征的症状。使用ZCD-Tipagem分子试剂盒的多重RT-qPCR证实了DENV-3感染，暗示一个可能的本土案件，因为患者报告没有旅行史。为了及时评估这种复苏，我们应用了纳米孔测序技术。这允许对2023年在米纳斯吉拉斯州分离的初始DENV-3病例进行快速表征，这代表了自血清型在该州先前记录的循环以来的13年间隔。本病例报告强调了积极监测和迅速实施有针对性的控制策略以应对登革热不断发展的动态的至关重要性。特别强调DENV-3在该州的复兴。
