PDF(Pubmed)
Abstract：
Phytophthora blight of pepper is a notorious disease caused by the oomycete pathogen Phytophthora capsici, which poses a great threat to global pepper production. MicroRNA (miRNA) is a class of non-coding small RNAs that regulate gene expressions by altering the translation efficiency or stability of targeted mRNAs, which play important roles in the regulation of a plant\'s response to pathogens. Herein, time-series mRNA-seq libraries and small RNA-seq libraries were constructed using pepper roots from the resistant line CM334 and the susceptible line EC01 inoculated with P. capsici at 0, 6, 24, and 48 h post-inoculation, respectively. For mRNA-seq analysis, a total of 2159 and 2971 differentially expressed genes (DEGs) were identified in CM334 and EC01, respectively. For miRNA-seq analysis, 491 pepper miRNAs were identified, including 330 known miRNAs and 161 novel miRNAs. Among them, 69 and 88 differentially expressed miRNAs (DEMs) were identified in CM334 and EC01, respectively. Examination of DEMs and their targets revealed 22 regulatory networks, predominantly featuring up-regulated miRNAs corresponding to down-regulated target genes. Notably, these DEM-DEG regulatory networks exhibited significant overlap between CM334 and EC01, suggesting that they might contribute to pepper\'s basal defense against P. capsici. Furthermore, five selected DEMs (miR166, miR1171, miR395, miR530 and miRN2) and their target genes underwent qRT-PCR validation, confirming a consistent negative correlation in the expression patterns of miRNAs and their targets. This comprehensive analysis provides novel insights into the regulatory networks of miRNAs and their targets, offering valuable contributions to our understanding of pepper\'s defense mechanisms against P. capsici.
摘要：
辣椒疫霉疫病是由卵菌病原体辣椒疫霉引起的臭名昭著的疾病，这对全球辣椒生产构成了巨大威胁。MicroRNA(miRNA)是一类非编码小RNA,通过改变靶mRNA的翻译效率或稳定性来调控基因表达。在调节植物对病原体的反应中起重要作用。在这里，时间序列mRNA-seq文库和小RNA-seq文库是使用来自抗性系CM334和易感系EC01的辣椒根构建的，接种后0、6、24和48小时，分别。对于mRNA-seq分析，在CM334和EC01中分别鉴定出2159和2971个差异表达基因（DEGs）。对于miRNA-seq分析，鉴定出491个辣椒miRNA,包括330个已知的miRNA和161个新的miRNA。其中,在CM334和EC01中分别鉴定了69和88个差异表达的miRNA(DEM)。对DEM及其目标的检查揭示了22个监管网络，主要表现为上调的miRNA对应于下调的靶基因。值得注意的是,这些DEM-DEG调控网络在CM334和EC01之间表现出明显的重叠，表明它们可能有助于辣椒对辣椒的基础防御。此外，五个选定的DEM（miR166，miR1171，miR395，miR530和miRN2）及其靶基因进行了qRT-PCR验证，证实miRNA及其靶标的表达模式存在一致的负相关。这种全面的分析为miRNA及其靶标的调控网络提供了新的见解。为我们理解辣椒对辣椒的防御机制提供了宝贵的贡献。
