PDF(Pubmed)
Abstract：
BACKGROUND: Candida albicans and similar species are significant pathogens in immunocompromised and hospitalized individuals, known for mucosal colonization and bloodstream/organ invasion. Many pathogenic fungi, including these species, exhibit autofluorescence (R/OF) under specific light conditions, a feature crucial for their detection.
OBJECTIVE: We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of Candida spp., common in the oral cavity, exploring its potential in health screenings.
METHODS: This study utilized cultures of Candida spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation.
RESULTS: Most Candida strains displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation.
CONCLUSIONS: The majority of the Candida strains tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for Candida spp.
OBJECTIVE: We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of Candida spp., common in the oral cavity, exploring its potential in health screenings.
METHODS: This study utilized cultures of Candida spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation.
RESULTS: Most Candida strains displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation.
CONCLUSIONS: The majority of the Candida strains tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for Candida spp.
摘要：
背景：白色念珠菌和类似物种是免疫受损和住院个体中的重要病原体，已知粘膜定植和血流/器官侵入。许多病原真菌，包括这些物种，在特定光照条件下表现出自发荧光(R/OF)，对他们的检测至关重要的特征。
目的：我们研究了使用405nm二极管激光直接观察念珠菌属的红色/橙色自发荧光。,常见于口腔，探索其在健康检查中的潜力。
方法：本研究利用念珠菌培养物。在Sabouraud葡萄糖琼脂上使用Qdot655和685进行荧光基准测试，使用405nm二极管激光器(连续波，功率250mW,0.0425J/cm²通量,0.0014W/cm²功率密度)。使用黄色滤光片相机以设定的间隔（48至144小时）捕获图像。视觉和计算分析根据存在来评估R/OF，强度,着色,和菌落内变异。
结果：大多数念珠菌菌株在所有观察时间均显示红色/橙色自发荧光，以不同的着色和菌落内分布为特征。最初，R/OF强度增加，然后在观察的后期稳定。
结论：测试的大多数念珠菌菌株能够在405nm激光下发射R/OF。这一发现为将R/OF检测整合到念珠菌的常规牙科筛查中开辟了新的可能性。
目的：我们研究了使用405nm二极管激光直接观察念珠菌属的红色/橙色自发荧光。,常见于口腔，探索其在健康检查中的潜力。
方法：本研究利用念珠菌培养物。在Sabouraud葡萄糖琼脂上使用Qdot655和685进行荧光基准测试，使用405nm二极管激光器(连续波，功率250mW,0.0425J/cm²通量,0.0014W/cm²功率密度)。使用黄色滤光片相机以设定的间隔（48至144小时）捕获图像。视觉和计算分析根据存在来评估R/OF，强度,着色,和菌落内变异。
结果：大多数念珠菌菌株在所有观察时间均显示红色/橙色自发荧光，以不同的着色和菌落内分布为特征。最初，R/OF强度增加，然后在观察的后期稳定。
结论：测试的大多数念珠菌菌株能够在405nm激光下发射R/OF。这一发现为将R/OF检测整合到念珠菌的常规牙科筛查中开辟了新的可能性。
