PDF(Pubmed)
Abstract：
Two-pore K+ (TPK) channels are voltage-independent and involved in stress response in plants. Herein, we identified 12 TaTPK genes located on nine chromosomes in the Triticum aestivum genome. The majority of TaTPK genes comprised two exons. Each TaTPK channel comprised four transmembrane (TM) helices, N- and C-terminal ion-channel domains, two EF-hand domains and one 14-3-3 binding site. Additionally, highly conserved \'GYGD\' motif responsible for K+ ion specificity, was found in between the TMs in both the ion-channel domains. Nine TaTPK channels were predicted to be localised at the plasma membrane, while three were vacuolar. The protein-protein and protein-chemical interactions indicated the coordinated functioning of the TaTPK channels with the other K+ transporters and their possible interaction with the Ca2+-signaling pathway. Expression studies suggested their importance in both vegetative and reproductive tissues development. Significantly modulated expression of various TaTPK genes during heat, drought, combined heat and drought and salt stresses, and after fungal infestation, depicted their function in stress responses. The miRNAs and transcription factors interaction analyses suggested their role in the hormone, light, growth and development-related, and stress-responsive signaling cascades. The current study suggested vital functions of various TaTPK genes, especially in stress response, and would provide an opportunity for their detailed characterization in future studies.
摘要：
双孔K（TPK）通道与电压无关，并参与植物的胁迫反应。在这里，我们确定了位于普通小麦基因组中9条染色体上的12个TaTPK基因。大多数TaTPK基因包含两个外显子。每个TaTPK通道包含四个跨膜（TM）螺旋，N-和C-末端离子通道域，两个EF-手结构域和一个14-3-3结合位点。此外,高度保守的“GYGD”基序负责K+离子特异性，在两个离子通道域的TM之间发现。预测有9个TaTPK通道位于质膜，三个是空泡的.蛋白质-蛋白质和蛋白质-化学相互作用表明TaTPK通道与其他K转运蛋白的协调功能以及它们与Ca2信号传导途径的可能相互作用。表达研究表明，它们在营养和生殖组织发育中都很重要。在加热过程中各种TaTPK基因的表达显着调节，干旱,热、干旱和盐胁迫相结合，真菌感染后，描绘了它们在应激反应中的功能。miRNAs和转录因子相互作用分析提示它们在激素中的作用,光,与成长和发展有关，和应激反应信号级联。目前的研究表明各种TaTPK基因的重要功能，尤其是在应激反应中，并将在未来的研究中为他们的详细表征提供机会。
