Abstract：
Besides being scarce, the drugs available for treating cutaneous leishmaniasis have many adverse effects. Ozone is an option to enhance the standard treatment due to the wound-healing activity reported in the literature. In this study, we evaluated the efficiency of ozonated sunflower oil as an adjuvant in treating cutaneous lesions caused by Leishmania amazonensis. BALB/c mice were infected with L. amazonensis, and after the lesions appeared, they were treated in four different schedules using the drug treatment with meglumine antimoniate (Glucantime®), with or without ozonated oil. After thirty days of treatment, the lesions\' thickness and their parasitic burden, blood leukocytes, production of NO and cytokines from peritoneal macrophages and lymph node cells were analyzed. The group treated with ozonated oil plus meglumine antimoniate showed the best performance, improving the lesion significantly. The parasitic burden showed that ozonated oil enhanced the leishmanicidal activity of the treatment, eliminating the parasites in the lesion. Besides, a decrease in the TNF levels from peritoneal macrophages and blood leukocytes demonstrated an immunomodulatory action of ozone in the ozonated oil-treated animals compared to the untreated group. Thus, ozonated sunflower oil therapy has been shown as an adjuvant in treating Leishmania lesions since this treatment enhanced the leishmanicidal and wound healing effects of meglumine antimoniate.
摘要：
除了稀缺，可用于治疗皮肤利什曼病的药物有许多副作用。由于文献中报道的伤口愈合活性,臭氧是增强标准治疗的一种选择。在这项研究中,我们评估了臭氧化葵花籽油作为佐剂治疗亚马逊利什曼原虫引起的皮肤病变的效率。BALB/c小鼠感染了亚马逊乳杆菌，病变出现后，他们在四种不同的方案中使用抗蒙酸葡甲胺（Glucoantime®）的药物治疗，有或没有臭氧化油。经过三十天的治疗，病变的厚度和它们的寄生负担，血液白细胞，分析了腹膜巨噬细胞和淋巴结细胞中NO和细胞因子的产生。用臭氧化油加锑酸葡甲胺治疗的组表现出最佳的性能，显著改善病变。寄生负荷表明，臭氧化油增强了治疗的利什曼杀活性，消除病变中的寄生虫。此外,来自腹膜巨噬细胞和血液白细胞的TNF水平降低表明臭氧在臭氧处理的动物中与未处理组相比具有免疫调节作用.因此，臭氧化葵花籽油疗法已被证明是治疗利什曼原虫病变的佐剂，因为这种治疗增强了乙酰葡胺的杀利什曼和伤口愈合作用。
