Abstract:
The objective of this study was to quantify metabolic compounds in leaves of A. niopoides and S. polyphylla and to evaluate the antitumor potential of extracts from both species in cervical tumour cells. The physiological analyses performed were quantification of starch, sucrose, phenolic compounds and proteins. An aqueous extract was prepared and added to the SiHa cell line at concentrations of 10, 100 and 1000 μg/mL at 4h, 24h, 48h and 72h. Cell morphology, proliferation and viability were analysed. The species showed a large amount of starch and phenolic compounds. Treatment with the extract of both species caused morphological changes in SiHa cells and exhibited antiproliferative effects at a concentration of 1000 µg/ml. In cell viability test, only A. niopoides showed a significant reduction. The study presented the effects of the species against a cervical cancer cell line, where A. niopoides has already shown to be a promising plant drug.
摘要:
这项研究的目的是量化牛位鱼和多叶草叶片中的代谢化合物,并评估两种物种提取物在宫颈肿瘤细胞中的抗肿瘤潜力。进行的生理分析是淀粉的定量,蔗糖,酚类化合物和蛋白质。制备水提取物,并在4小时以10、100和1000μg/mL的浓度添加到SiHa细胞系中,24h,48h和72h。细胞形态学,分析了增殖和活力。该物种显示了大量的淀粉和酚类化合物。用两种物种的提取物处理导致SiHa细胞的形态变化,并在1000µg/ml的浓度下表现出抗增殖作用。在细胞活力测试中,只有A.niopoides显示显着减少。该研究展示了该物种对宫颈癌细胞系的影响,其中A.niopoides已经被证明是一种有前途的植物药物。