PDF(Pubmed)
Abstract：
Gallstones are crystalline deposits in the gallbladder that are traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota and host metabolism variances among the different types of gallstones remain largely unclear. Here, the bile and gallstone microbial species spectra of 29 subjects with gallstone disease (GSD, 24 cholesterol and 5 pigment) were revealed by type IIB restriction site-associated DNA microbiome sequencing (2bRAD-M). Among them (21 subjects: 18 cholesterol and 3 pigment), plasma samples were subjected to liquid chromatography-mass spectrometry (LC-MS) untargeted metabolomics. The microbiome yielded 896 species comprising 882 bacteria, 13 fungi, and 1 archaeon. Microbial profiling revealed significant enrichment of Cutibacterium acnes and Microbacterium sp005774735 in gallstone and Agrobacterium pusense and Enterovirga sp013044135 in the bile of cholesterol GSD subjects. The metabolome revealed 2296 metabolites, in which malvidin 3-(6\'\'-malonylglucoside), 2-Methylpropyl glucosinolate, and ergothioneine were markedly enriched in cholesterol GSD subjects. Metabolite set enrichment analysis (MSEA) demonstrated enriched bile acids biosynthesis in individuals with cholesterol GSD. Overall, the multi-omics analysis revealed that microbiota and host metabolism interaction perturbations differ depending on the disease type. Perturbed gallstone type-related microbiota may contribute to unbalanced bile acids metabolism in the gallbladder and host, representing a potential early diagnostic marker and therapeutic target for GSD.
摘要：
胆结石是胆囊中的结晶沉积物，传统上被归类为胆固醇，颜料,或根据其成分混合的石头。不同类型胆结石之间的微生物区系和宿主代谢差异仍不清楚。这里,29名胆结石患者的胆汁和胆结石微生物物种谱(GSD，24胆固醇和5色素）通过IIB型限制性位点相关的DNA微生物组测序（2bRAD-M）揭示。其中（21名受试者：18胆固醇和3色素），血浆样品进行液相色谱-质谱(LC-MS)非靶向代谢组学检测.微生物组产生了896个物种，包括882个细菌，13种真菌，1个古细菌。微生物谱分析显示，在胆固醇GSD受试者的胆汁中，胆结石和农杆菌中的痤疮杆菌和Microbacteriumsp005774735以及Enterovirgasp013044135显着富集。代谢组显示2296种代谢物，其中malvidin3-（6\'\'-丙二酰葡萄糖苷），2-甲基丙基芥子油苷，和麦角硫因在胆固醇GSD受试者中明显富集。代谢物集富集分析（MSEA）证明了胆固醇GSD个体中富含胆汁酸的生物合成。总的来说,多组学分析显示,微生物群和宿主代谢相互作用扰动因疾病类型而异.受干扰的胆结石类型相关微生物群可能导致胆囊和宿主中胆汁酸代谢不平衡，代表GSD的潜在早期诊断标志物和治疗靶标。
