PDF(Pubmed)
Abstract：
Taraxacum mongolicum (TM) is a kind of medicinal and edible homologous plant which is included in the catalogue of feed raw materials in China. It is rich in polyphenols, flavonoids, polysaccharides and other active substances, and shows many benefits to livestock, poultry and aquatic products. The study aimed to assess the potential of TM aqueous extract (TMAE) as a substitute for poultry AGPs.
A total of 240 one-day-old Arbor Acker broilers were randomly assigned to four groups and fed a basal diet (Con) supplemented with 500, 1000, and 2000 mg/kg TMAE (Low, Medium, and High groups). The growth performance of the broilers was measured on day 21 and day 42. At the end of the trial, the researchers measured slaughter performance and collected serum, liver, spleen, ileum, and intestinal contents to investigate the effects of TMAE on serum biochemistry, antioxidant capacity, immune function, organ coefficient, intestinal morphology, flora composition, and short-chain fatty acids (SCFAs).
The results showed that broilers treated with TMAE had a significantly higher average daily gain from 22 to 42 days old compared to the Con group. Various doses of TMAE resulted in different levels of improvement in serum chemistry. High doses increased serum alkaline phosphatase and decreased creatinine. TMAE also increased the antioxidant capacity of serum, liver, and ileum in broilers. Additionally, middle and high doses of TMAE enhanced the innate immune function of the liver (IL-10) and ileum (Occludin) in broilers. Compared to the control group, the TMAE treatment group exhibited an increase in the ratio of villi length to villi crypt in the duodenum. TMAE increased the abundance of beneficial bacteria, such as Alistipes and Lactobacillus, while reducing the accumulation of harmful bacteria, such as Colidextracter and Sellimonas. The cecum\'s SCFAs content increased with a medium dose of TMAE. Supplementing broiler diets with TMAE at varying doses enhanced growth performance and overall health. The most significant benefits were observed at a dose of 1000 mg/kg, including improved serum biochemical parameters, intestinal morphology, antioxidant capacity of the liver and ileum, immune function of the liver and ileum, and increased SCFAs content. Lactobacillus aviarius, norank_f_norank_o__Clostridia_UCG-014, and Flavonifractor are potentially dominant members of the intestinal microflora.
In conclusion, TMAE is a promising poultry feed additive and 1000 mg/kg is an effective reference dose.
A total of 240 one-day-old Arbor Acker broilers were randomly assigned to four groups and fed a basal diet (Con) supplemented with 500, 1000, and 2000 mg/kg TMAE (Low, Medium, and High groups). The growth performance of the broilers was measured on day 21 and day 42. At the end of the trial, the researchers measured slaughter performance and collected serum, liver, spleen, ileum, and intestinal contents to investigate the effects of TMAE on serum biochemistry, antioxidant capacity, immune function, organ coefficient, intestinal morphology, flora composition, and short-chain fatty acids (SCFAs).
The results showed that broilers treated with TMAE had a significantly higher average daily gain from 22 to 42 days old compared to the Con group. Various doses of TMAE resulted in different levels of improvement in serum chemistry. High doses increased serum alkaline phosphatase and decreased creatinine. TMAE also increased the antioxidant capacity of serum, liver, and ileum in broilers. Additionally, middle and high doses of TMAE enhanced the innate immune function of the liver (IL-10) and ileum (Occludin) in broilers. Compared to the control group, the TMAE treatment group exhibited an increase in the ratio of villi length to villi crypt in the duodenum. TMAE increased the abundance of beneficial bacteria, such as Alistipes and Lactobacillus, while reducing the accumulation of harmful bacteria, such as Colidextracter and Sellimonas. The cecum\'s SCFAs content increased with a medium dose of TMAE. Supplementing broiler diets with TMAE at varying doses enhanced growth performance and overall health. The most significant benefits were observed at a dose of 1000 mg/kg, including improved serum biochemical parameters, intestinal morphology, antioxidant capacity of the liver and ileum, immune function of the liver and ileum, and increased SCFAs content. Lactobacillus aviarius, norank_f_norank_o__Clostridia_UCG-014, and Flavonifractor are potentially dominant members of the intestinal microflora.
In conclusion, TMAE is a promising poultry feed additive and 1000 mg/kg is an effective reference dose.
摘要：
■蒲公英（TM）是一种药食同源植物，已列入中国饲料原料目录。它富含多酚，黄酮类化合物，多糖和其他活性物质，对牲畜有很多好处，家禽和水产品。该研究旨在评估TM水提取物（TMAE）作为家禽AGP替代品的潜力。
■将总共240只一天龄的ArborAcker肉鸡随机分为四组，并饲喂补充500、1000和2000mg/kgTMAE（低，中等,和高级团体）。在第21天和第42天测量肉鸡的生长性能。审判结束时,研究人员测量了屠宰性能并收集了血清，肝脏,脾,脾回肠,和肠道内容物，以研究TMAE对血清生化的影响，抗氧化能力，免疫功能，器官系数，肠道形态学,植物区系组成,和短链脂肪酸（SCFA）。
■结果表明，与Con组相比，用TMAE处理的肉鸡从22至42天龄的平均日增重明显更高。不同剂量的TMAE导致不同水平的血清化学改善。高剂量血清碱性磷酸酶升高，肌酐降低。TMAE还增加了血清的抗氧化能力，肝脏,和肉鸡的回肠。此外,中高剂量的TMAE增强了肉仔鸡肝脏（IL-10）和回肠（Occludin）的先天免疫功能。与对照组相比,TMAE治疗组表现出十二指肠中绒毛长度与绒毛隐窝的比率增加。TMAE增加了有益细菌的丰度，如Alistipes和乳酸菌，同时减少有害细菌的积累，如Colidextractter和Sellimonas。盲肠SCFAs含量随着中等剂量TMAE的增加而增加。用不同剂量的TMAE补充肉鸡日粮可增强生长性能和整体健康。在1000mg/kg的剂量下观察到最显著的益处，包括改善血清生化指标,肠道形态学,肝脏和回肠的抗氧化能力，肝脏和回肠的免疫功能，SCFA含量增加。鸟乳酸杆菌,norank_f_norank_o__Clostridia_UCG-014和Flavonifractor是肠道菌群的潜在优势成员。
■总而言之，TMAE是一种有前途的家禽饲料添加剂，1000mg/kg是有效的参考剂量。
■将总共240只一天龄的ArborAcker肉鸡随机分为四组，并饲喂补充500、1000和2000mg/kgTMAE（低，中等,和高级团体）。在第21天和第42天测量肉鸡的生长性能。审判结束时,研究人员测量了屠宰性能并收集了血清，肝脏,脾,脾回肠,和肠道内容物，以研究TMAE对血清生化的影响，抗氧化能力，免疫功能，器官系数，肠道形态学,植物区系组成,和短链脂肪酸（SCFA）。
■结果表明，与Con组相比，用TMAE处理的肉鸡从22至42天龄的平均日增重明显更高。不同剂量的TMAE导致不同水平的血清化学改善。高剂量血清碱性磷酸酶升高，肌酐降低。TMAE还增加了血清的抗氧化能力，肝脏,和肉鸡的回肠。此外,中高剂量的TMAE增强了肉仔鸡肝脏（IL-10）和回肠（Occludin）的先天免疫功能。与对照组相比,TMAE治疗组表现出十二指肠中绒毛长度与绒毛隐窝的比率增加。TMAE增加了有益细菌的丰度，如Alistipes和乳酸菌，同时减少有害细菌的积累，如Colidextractter和Sellimonas。盲肠SCFAs含量随着中等剂量TMAE的增加而增加。用不同剂量的TMAE补充肉鸡日粮可增强生长性能和整体健康。在1000mg/kg的剂量下观察到最显著的益处，包括改善血清生化指标,肠道形态学,肝脏和回肠的抗氧化能力，肝脏和回肠的免疫功能，SCFA含量增加。鸟乳酸杆菌,norank_f_norank_o__Clostridia_UCG-014和Flavonifractor是肠道菌群的潜在优势成员。
■总而言之，TMAE是一种有前途的家禽饲料添加剂，1000mg/kg是有效的参考剂量。
