PDF(Pubmed)
Abstract：
Waterfowl astroviruses are mainly duck astroviruses and goose astroviruses, of which duck astroviruses (DAstV-3, -4), goose astroviruses (GoAstV-1, -2) are the four new waterfowl 21 astroviruses in recent years, which can lead to enteritis, viral hepatitis, gout and reduce the growth performance of waterfowl, affecting the healthy development of the waterfowl farming industry. Since no targeted drugs or vaccines on the market, studies on the epidemiology of the virus are necessary for vaccine development. In this study, we collected 1546 waterfowl samples from 13 provinces in China for epidemiological investigation. The results showed that 260 samples (16.8%) were positive. Four species of astrovirus were detected in 13 provinces except Fujian province. Among the four sites tested, the highest positive rates were found in farms and slaughterhouses. Cross-host and mixed infection were observed in four species of waterfowl astroviruses. The whole genome of 17 isolates was sequenced and compared with published sequences. Genetic evolution and homology analysis showed that the isolated strains had high similarity to their reference sequences. To assess the pathogenicity of GoAstV, 7-day-old goslings were inoculated with GoAstV-1 and GoAstV-2 by the intramuscular route, and infected geese showed similar clinical signs, such as anorexia, depression, and weight loss. Organ damage was seen after infection, with histopathological changes in the heart, liver, spleen, kidney, and intestine, and higher viral loads in throat and anal swabs. These findings increase our understanding of the pathogenicity of GoAstV-1 and GoAstV-2 in goslings and provide more references for future research.
摘要：
水禽星状病毒主要有鸭星状病毒和鹅星状病毒,其中鸭星状病毒（DAstV-3，-4），鹅星状病毒(GoAstV-1,-2)是近年来发现的四种新型水禽星状病毒,会导致肠炎,病毒性肝炎,痛风和降低水禽的生长性能，影响水禽养殖业的健康发展。由于市场上没有靶向药物或疫苗，病毒的流行病学研究对于疫苗开发是必要的。在这项研究中,我们收集了中国13个省份的1546份水禽样本进行流行病学调查。结果显示260份(16.8%)样品为阳性。除福建省外，13个省共检测到4种星状病毒。在测试的四个地点中，阳性率最高的是农场和屠宰场。在4种水禽星状病毒中观察到跨宿主和混合感染。对17个分离株的全基因组进行了测序，并与已发表的序列进行了比较。遗传进化和同源性分析表明，分离菌株与参考序列具有较高的相似性。为了评估GoAstV的致病性，7日龄小鹅通过肌内途径接种GoAstV-1和GoAstV-2,感染的鹅也表现出相似的临床症状,如厌食症，抑郁症,和减肥。感染后可见器官损伤，心脏的组织病理学改变，肝脏,脾,脾肾,和肠，喉咙和肛门拭子中的病毒载量更高。这些发现增加了我们对GoAstV-1和GoAstV-2在鹅中的致病性的认识，并为今后的研究提供了更多的参考。
