Pneumonia Caused by Chlamydia psittaci and SARS-CoV-2 Coinfection Diagnosed Using Metagenomic Next-Generation Sequencing: A Case Report.

使用宏基因组下一代测序诊断的鹦鹉衣原体和 SARS - CoV - 2 共感染引起的肺炎 ： 病例报告。

• 发表时间: 2024
• 来源期刊: Int Med Case Rep J
• DOI: 10.2147/IMCRJ.S458131
• PMID: 38529114
• 文章类型: Case Reports
• 作者列表: Zhang A,Liang J,Lao X,Xia X,Liang J

关键词: bronchoalveolar lavage fluid; coinfection; quantitative reverse-transcription polymerase chain reaction; zoonoses

Abstract

We report a case of pneumonia caused by coinfection with Chlamydia psittaci and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron XBB.1 variant, confirmed using metagenomic next-generation sequencing (mNGS) and quantitative polymerase chain reaction (qPCR). C. psittaci and SARS-CoV-2 were detected in bronchoalveolar lavage fluid using mNGS. Additionally, mNGS detected C. psittaci in blood and nasopharyngeal specimens and was more sensitive than qPCR. The patient recovered after treatment with moxifloxacin. This report highlights the use of coinfections of C. psittaci and SARS-CoV-2, as mNGS has already been recognized to be a diagnostic tool for identifying coinfections.

摘要

我们报告了一例由鹦鹉衣原体和严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）OmicronXBB.1变体共同感染引起的肺炎，使用宏基因组下一代测序（mNGS）和定量聚合酶链反应（qPCR）确认。使用mNGS在支气管肺泡灌洗液中检测到鹦鹉和SARS-CoV-2。此外,mNGS检测到血液和鼻咽标本中的鹦鹉，比qPCR更敏感。患者在接受莫西沙星治疗后康复。该报告重点介绍了鹦鹉和SARS-CoV-2共感染的使用，因为mNGS已经被认为是识别共感染的诊断工具。