PDF(Pubmed)
Abstract：
One factor for the lacking integration of the middle ear stapes footplate prosthesis or the missing healing of stapes footplate fractures could be the known osteogenic inactivity. In contrast, it was recently demonstrated that titanium prostheses with an applied collagen matrix and immobilised growth factors stimulate osteoblastic activation and differentiation on the stapes footplate. Regarding those findings, the aim of this study was to evaluate the potential of bone regeneration including bone remodeling in the middle ear. Ten one-year-old female merino sheep underwent a middle ear surgery without implantation of middle ear prostheses or any other component for activating bone formation. Post-operatively, four fluorochromes (tetracycline, alizarin complexion, calcein green and xylenol orange) were administered by subcutaneous injection at different time points after surgery (1 day: tetracycline, 7 days: alizarin, 14 days: calcein, 28 days: xylenol). After 12 weeks, the temporal bones including the lateral skull base were extracted and histologically analyzed. Fluorescence microscopy analysis of the entire stapes with the oval niche, but in particular stapes footplate and the Crura stapedis revealed evidence of new bone formation. Calcein was detected in all and xylenol in 60% of the animals. In contrast, tetracycline and alizarin could only be verified in two animals. The authors were able to demonstrate the osseoregenerative potential of the middle ear, in particular of the stapes footplate, using fluorescence sequence labelling.
摘要：
中耳骨足板假体缺乏整合或骨足板骨折愈合缺失的一个因素可能是已知的成骨不活动。相比之下,最近证明，具有应用胶原基质和固定生长因子的钛假体可刺激骨骨板上的成骨细胞活化和分化。关于这些发现,这项研究的目的是评估中耳骨再生的潜力，包括骨重建。十只一岁的雌性美利奴羊接受了中耳手术，没有植入中耳假体或任何其他激活骨形成的组件。术后,四种荧光染料（四环素，茜素肤色,在手术后的不同时间点皮下注射钙绿和二甲酚橙）（1天：四环素，7天:茜素,14天:钙黄绿素,28天：二甲苯酚）。12周后,提取颞骨，包括侧颅底，并进行组织学分析。荧光显微镜分析整个骨与椭圆形生态位，但特别是stapes骨板和Crurastapedis揭示了新骨形成的证据。在所有动物中检测到钙黄绿素，在60％的动物中检测到二甲酚。相比之下,四环素和茜素只能在两只动物中验证。作者能够证明中耳的骨生成潜力，特别是stapes脚踏板，使用荧光序列标记。
