Abstract：
The response patterns of microbial functional genes involved in biogeochemical cycles to cadaver decay is a central topic of recent environmental sciences. However, the response mechanisms and pathways of the functional genes associated with the carbon (C) and nitrogen (N) cycling to cadaveric substances such as cadaverine and putrescine remain unclear. This study explored the variation of functional genes associated with C fixation, C degradation and N cycling and their influencing factors under cadaverine, putrescine and mixed treatments. Our results showed only putrescine significantly increased the alpha diversity of C fixation genes, while reducing the alpha diversity of N cycling genes in sediment. For the C cycling, the mixed treatment significantly decreased the total abundance of reductive acetyl-CoA pathway genes (i.e., acsB and acsE) and lig gene linked to lignin degradation in water, while only significantly increasing the hydroxypropionate-hydroxybutylate cycle (i.e., accA) gene abundance in sediment. For the N cycling, mixed treatment significantly decreased the abundance of the nitrification (i.e., amoB), denitrification (i.e., nirS3) genes in water and the assimilation pathway gene (i.e., gdhA) in sediment. Environmental factors (i.e., total carbon and total nitrogen) were all negatively associated with the genes of C and N cycling. Therefore, cadaverine and putrescine exposure may inhibit the pathway in C fixation and N cycling, while promoting C degradation. These findings can offer some new insight for the management of amine pollution caused by animal cadavers.
摘要：
参与生物地球化学循环的微生物功能基因对尸体腐烂的响应模式是最近环境科学的中心主题。然而,与碳（C）和氮（N）循环相关的功能基因对尸体物质如尸体和腐胺的反应机制和途径尚不清楚。本研究探索了与C固定相关的功能基因的变异，尸胺下碳降解和氮循环及其影响因素,腐胺和混合治疗。我们的结果表明，只有腐胺显着增加了C固定基因的α多样性，同时降低了沉积物中N循环基因的α多样性。对于C循环，混合处理显着降低了还原性乙酰辅酶A途径基因的总丰度(即，acsB和acsE）和lig基因与水中木质素降解有关，而仅显着增加羟基丙酸酯-羟基丁酸酯循环(即，accA）沉积物中的基因丰度。对于N循环，混合处理显著降低了硝化的丰度(即，amoB)，反硝化(即，nirS3)水中的基因和同化途径基因(即，gdhA）在沉积物中。环境因素(即，总碳和总氮）都与C和N循环的基因负相关。因此,尸胺和腐胺暴露可能会抑制C固定和N循环的途径，同时促进C降解。这些发现可以为动物尸体引起的胺污染的管理提供一些新的见解。
