Abstract：
Green tea extract (GTE) contains antioxidants that are present in green tea. The active constituents of green tea extract are catechins. This study demonstrates a spectrofluorimetric method for measuring GTE\'s catechin concentration based on its native fluorescence. To design a quick, sensitive, and ecological spectrofluorimetric approach, all features were investigated and adjusted. This method relies on determining the GTE ethanolic solution\'s native fluorescence at 312 nm after excitation at 227 nm. The calibration graph displayed a linear regression for values between 0.05 and 1.0 μg mL-1. The detection and quantification limits of the proposed technique were 0.008 and 0.026 μg mL-1, respectively. Two pure catechins present in GTE, (-)-epicatechin and (-)-epigallocatechin gallate, were examined by the proposed method. The analytical estimation of GTE in the pharmaceutical tablet was achieved effectively using this approach. An adequate degree of agreement was found when the findings were compared to those obtained by the comparative technique. Therefore, the novel strategy may be used in the GTE quality control study with minimal risks to people or the environment. The quantum yields of catechins were estimated. The validated technique was accepted by the International Council of Harmonization criteria.
摘要：
绿茶提取物（GTE）含有绿茶中存在的抗氧化剂。绿茶提取物的活性成分是儿茶素。这项研究证明了一种基于天然荧光测量GTE儿茶素浓度的荧光光谱法。为了设计一个快速的，敏感,和生态荧光光谱法，对所有特征进行了调查和校正.该方法依赖于在227nm激发后在312nm确定GTE乙醇溶液的天然荧光。校准图显示0.05至1.0μgmL-1之间的值的线性回归。该技术的检测限和定量限分别为0.008和0.026μgmL-1。GTE中存在两种纯儿茶素，（-）-表儿茶素和（-）-表没食子儿茶素没食子酸酯，用所提出的方法进行了检验。使用该方法有效地实现了药物片剂中GTE的分析估计。当将调查结果与通过比较技术获得的结果进行比较时，发现了足够的一致性。因此,该新策略可用于GTE质量控制研究,且对人或环境的风险最小.估计了儿茶素的量子产率。经过验证的技术已被国际协调理事会标准接受。
