Abstract：
Flow cytometry is a critical technology for biomedical analysis and is an essential component of almost any study of the immune system. Widespread usage and increasing instrument complexity have, however, led to increasing neglect of education in their basic operating principles, a common situation with many technologies. This chapter describes the basics of flow cytometer operation using the Make Your Own Flow Cytometer ( https://www.cytometryworks.com ), a working cytometer than can be assembled by students into a functional instrument. This project and others like it is seeing widespread usage in biomedical education and can serve as models for like-minded investigators who wish to build their own systems. They also provide a good mechanism to introduce the key operational principles of flow cytometry as illustrated here.
摘要：
流式细胞术是生物医学分析的关键技术，几乎是任何免疫系统研究的重要组成部分。广泛的使用和不断增加的仪器复杂性，然而,导致人们越来越忽视教育的基本操作原则，许多技术的常见情况。本章介绍使用MakeYourOwn流式细胞仪（https://www.cytometryworks.com)，可以由学生组装成功能性仪器的有效细胞仪。这个项目和其他类似的项目在生物医学教育中得到了广泛的应用，可以作为志同道合的研究者的模型，他们希望建立自己的系统。它们还提供了良好的机制来引入流式细胞术的关键操作原理,如本文所示。
