Abstract:
Bacteriophages are ubiquitous biological entities which can be found in a variety of habitats. Here, we describe protocols for the isolation of bacteriophages on a variety of Actinobacterial genera. Two approaches to phage isolation, direct isolation and enriched isolation, are described, which can be performed individually or in parallel. The protocols described can be adapted to isolate a wide array of bacteriophages.
摘要:
噬菌体是普遍存在的生物实体,可以在各种栖息地中找到。这里,我们描述了在各种放线菌属上分离噬菌体的方案。两种方法来分离噬菌体,直接隔离和富集隔离,被描述,可以单独或并行执行。所描述的方案可以适用于分离广泛的噬菌体。