PDF(Pubmed)
Abstract：
One of the most prevalent malignancies that significantly affects world health is colorectal cancer (CRC). While genetics are involved in a portion of CRC patients, most cases are sporadic. The microbiome composition could be a new source of tumor initiation and progression. This research was conducted to investigate the microbiota composition of CRC patients post colectomy at taxonomic and functional levels. Using a next-generation sequencing approach, using an Illumina Novaseq 6000, the fecal samples of 13 patients were analyzed and the obtained data was subjected to a bioinformatics analysis. The bacterial abundance and uniqueness varied in CRC patients alongside differences in bacterial counts between patients. Bacteroides fragilis, Bacteroides vulgatus, Escherichia coli, and Fusobacterium nucleatum were among the pro-cancerous microorganisms found. Concurrently, bacteria linked to CRC progression were detected that have been previously linked to metastasis and recurrence. At the same time, probiotic bacteria such as Bifidobacterium dentium, Bifidobacterium bifidum, and Akkermansia muciniphila increased in abundance after colectomies. Additionally, numerous pathways were deferentially enriched in CRC, which emerged from functional pathways based on bacterial shotgun data. CRC-specific microbiome signatures include an altered bacterial composition. Our research showed that microbial biomarkers could be more usefully employed to explore the link between gut microbiota and CRC using metagenomic techniques in the diagnosis, prognosis, and remission of CRC, thereby opening new avenues for CRC treatment.
摘要：
显著影响世界健康的最普遍的恶性肿瘤之一是结肠直肠癌(CRC)。虽然遗传学涉及一部分CRC患者，大多数病例是零星的。微生物组组成可能是肿瘤发生和发展的新来源。进行这项研究是为了在分类和功能水平上调查结肠切除术后CRC患者的微生物群组成。使用下一代测序方法，使用IlluminaNovaseq6000对13例患者的粪便样本进行了分析,并对获得的数据进行了生物信息学分析.CRC患者的细菌丰度和独特性以及患者之间细菌计数的差异。脆弱拟杆菌,普通拟杆菌,大肠杆菌,核梭杆菌是发现的促癌微生物之一。同时,检测到与CRC进展相关的细菌,这些细菌先前与转移和复发相关.同时,益生菌，如双歧杆菌，双歧杆菌，结肠切除术后，Akkermansia粘液病的丰度增加。此外,许多途径在CRC中被优先富集，它来自基于细菌猎枪数据的功能途径。CRC特异性微生物组特征包括改变的细菌组成。我们的研究表明，在诊断中使用宏基因组技术，微生物生物标志物可以更有效地用于探索肠道微生物群和CRC之间的联系。预后,和CRC的缓解，从而为CRC治疗开辟了新的途径。
