PDF(Pubmed)
Abstract：
Hemoglobin variants are often discovered when hemoglobin A1c (HbA1c) levels measured with a high-performance liquid chromatography (HPLC) system in fast mode are found to be low. The HA-8180V HPLC analyzer by Arkray offers two measurement modes: fast mode (FM) and variant mode (VM). Two Japanese patients with α-chain variant Hb Q-Iran detected incidentally after analyses with the HA-8180V in VM showed an abnormal peak, are presented. The first patient was a man in his 70 s, and the second patient was a man in his 50 s. Both were non-diabetic, but their results from HbA1c measurement in VM showed an abnormal peak. The VM-HbA1c, FM-HbA1c, and HbA1c measured by enzymatic assay and glycated albumin levels of the two patients were all within the reference ranges. They were diagnosed as having Hb Q-Iran (α2-75Asp → His) by globin gene analysis. It is difficult to detect α-chain hemoglobin variants based on abnormal FM-HbA1c levels, but measuring HbA1c in VM is useful for efficiently detecting hemoglobin variants.
摘要：
当发现以快速模式使用高效液相色谱（HPLC）系统测量的血红蛋白A1c（HbA1c）水平低时，通常会发现血红蛋白变体。Arkray的HA-8180VHPLC分析仪提供两种测量模式：快速模式（FM）和变体模式（VM）。两名患有α链变异HbQ-伊朗的日本患者在对VM中的HA-8180V进行分析后偶然发现了异常峰，被呈现。第一个病人是70多岁的男人,第二个病人是50多岁的男性。两人都没有糖尿病，但他们在VM的HbA1c测量结果显示出异常峰值。VM-HbA1c,FM-HbA1c,2例患者通过酶法测定的HbA1c和糖化白蛋白水平均在参考范围内。通过珠蛋白基因分析，他们被诊断为患有HbQ-伊朗（α2-75Asp→His）。很难根据FM-HbA1c水平异常检测α链血红蛋白变体，但是在VM中测量HbA1c对于有效检测血红蛋白变体是有用的。
