PDF(Pubmed)
Abstract：
UNASSIGNED: Reliable animal models are critical for preclinical research and should closely mimic the disease. With respect to route of infection, pathogenic agent, disease progression, clinical signs, and histopathological changes. Sheep have similar bone micro- and macrostructure as well as comparable biomechanical characteristics to humans. Their use in bone research is established, however their use in bone infection research is limited. This systematic review will summarise the key features of the available bone infection models using sheep, providing a reference for further development, validation, and application.
UNASSIGNED: This systematic review was designed according to the PRISMA guidelines and registered with PROSPERO. Quality was assessed using SYRICLE\'s risk of bias tool adapted for animal studies. PubMed, MEDLINE, Web of Science and EMBASE were searched until March 2022.1921 articles were screened by two independent reviewers, and 25 were included for analysis.
UNASSIGNED: Models have been developed in nine different breeds. Staphylococcus aureus was used in the majority of models, typically inoculating 108 colony forming units in tibial or femoral cortical defects. Infection was established with either planktonic or biofilm adherent bacteria, with or without foreign material implanted. Most studies used both radiological and microbiological analyses to confirm osteomyelitis.
UNASSIGNED: There is convincing evidence supporting the use of sheep in bone infection models of clinical disease. The majority of sheep studied demonstrated convincing osteomyelitis and tolerated the infection with minimal complications. Furthermore, the advantages of comparable biology and biomechanics may increase the success for translating in vivo results to successful therapies.
UNASSIGNED: In the realm of preclinical research, the translation to viable clinical therapies is often perilous, and the quest for reliable and representative animal models remains paramount. This systematic review accentuates the largely untapped potential of sheep as large animal models, especially in bone infection research. The anatomical and biomechanical parallels between sheep and human bone structures position sheep as an invaluable asset for studying osteomyelitis and periprosthetic joint infection. This comprehensive exploration of the literature demonstrates the robustness and translational promise of these models. Furthermore, this article underscores the potential applicability for sheep in developing effective therapeutic strategies for human bone infections.
UNASSIGNED: This systematic review was designed according to the PRISMA guidelines and registered with PROSPERO. Quality was assessed using SYRICLE\'s risk of bias tool adapted for animal studies. PubMed, MEDLINE, Web of Science and EMBASE were searched until March 2022.1921 articles were screened by two independent reviewers, and 25 were included for analysis.
UNASSIGNED: Models have been developed in nine different breeds. Staphylococcus aureus was used in the majority of models, typically inoculating 108 colony forming units in tibial or femoral cortical defects. Infection was established with either planktonic or biofilm adherent bacteria, with or without foreign material implanted. Most studies used both radiological and microbiological analyses to confirm osteomyelitis.
UNASSIGNED: There is convincing evidence supporting the use of sheep in bone infection models of clinical disease. The majority of sheep studied demonstrated convincing osteomyelitis and tolerated the infection with minimal complications. Furthermore, the advantages of comparable biology and biomechanics may increase the success for translating in vivo results to successful therapies.
UNASSIGNED: In the realm of preclinical research, the translation to viable clinical therapies is often perilous, and the quest for reliable and representative animal models remains paramount. This systematic review accentuates the largely untapped potential of sheep as large animal models, especially in bone infection research. The anatomical and biomechanical parallels between sheep and human bone structures position sheep as an invaluable asset for studying osteomyelitis and periprosthetic joint infection. This comprehensive exploration of the literature demonstrates the robustness and translational promise of these models. Furthermore, this article underscores the potential applicability for sheep in developing effective therapeutic strategies for human bone infections.
摘要：
■可靠的动物模型是临床前研究的关键，应该密切模拟疾病。关于感染途径，病原体，疾病进展,临床体征,和组织病理学变化。绵羊具有与人类相似的骨骼微观和宏观结构以及可比的生物力学特征。它们在骨骼研究中的应用已经确立，然而，它们在骨感染研究中的应用是有限的。本系统综述将总结使用绵羊的可用骨感染模型的关键特征，为进一步发展提供参考，验证,和应用。
■该系统综述是根据PRISMA指南设计的，并在PROSPERO注册。使用适用于动物研究的SYRICLE的偏倚风险工具评估质量。PubMed,MEDLINE,WebofScience和EMBASE在2022.22年3月之前进行了搜索，1921篇文章由两名独立审稿人筛选，25例纳入分析.
■已经在九个不同的品种中开发了模型。金黄色葡萄球菌在大多数模型中使用，通常在胫骨或股骨皮质缺损中接种108个菌落形成单位。感染是由浮游或生物膜粘附细菌建立的，有或没有异物植入。大多数研究使用放射学和微生物学分析来确认骨髓炎。
■有令人信服的证据支持在临床疾病的骨感染模型中使用绵羊。所研究的大多数绵羊表现出令人信服的骨髓炎，并以最小的并发症耐受感染。此外，可比较的生物学和生物力学的优势可能会增加将体内结果转化为成功疗法的成功率。
■在临床前研究领域，转化为可行的临床疗法通常是危险的，寻求可靠和有代表性的动物模型仍然至关重要。本系统综述强调了绵羊作为大型动物模型的未开发潜力，尤其是骨感染研究。绵羊和人类骨骼结构之间的解剖学和生物力学相似之处将绵羊定位为研究骨髓炎和假体周围关节感染的宝贵资产。对文献的全面探索证明了这些模型的鲁棒性和翻译前景。此外，本文强调了绵羊在开发人类骨感染的有效治疗策略方面的潜在适用性。
■该系统综述是根据PRISMA指南设计的，并在PROSPERO注册。使用适用于动物研究的SYRICLE的偏倚风险工具评估质量。PubMed,MEDLINE,WebofScience和EMBASE在2022.22年3月之前进行了搜索，1921篇文章由两名独立审稿人筛选，25例纳入分析.
■已经在九个不同的品种中开发了模型。金黄色葡萄球菌在大多数模型中使用，通常在胫骨或股骨皮质缺损中接种108个菌落形成单位。感染是由浮游或生物膜粘附细菌建立的，有或没有异物植入。大多数研究使用放射学和微生物学分析来确认骨髓炎。
■有令人信服的证据支持在临床疾病的骨感染模型中使用绵羊。所研究的大多数绵羊表现出令人信服的骨髓炎，并以最小的并发症耐受感染。此外，可比较的生物学和生物力学的优势可能会增加将体内结果转化为成功疗法的成功率。
■在临床前研究领域，转化为可行的临床疗法通常是危险的，寻求可靠和有代表性的动物模型仍然至关重要。本系统综述强调了绵羊作为大型动物模型的未开发潜力，尤其是骨感染研究。绵羊和人类骨骼结构之间的解剖学和生物力学相似之处将绵羊定位为研究骨髓炎和假体周围关节感染的宝贵资产。对文献的全面探索证明了这些模型的鲁棒性和翻译前景。此外，本文强调了绵羊在开发人类骨感染的有效治疗策略方面的潜在适用性。
