UNASSIGNED: In a cross-sectional study, a real-time PCR was carried out using HiTeq. 17 Viro Respiratory pathogen One Step RT-PCR Kit (Genova, Bonda Faravar, Bioluence, Tehran, Iran).
UNASSIGNED: A total of 311 individuals (mean age ± SD: 48.2 ± 21.7 years, range: 1-97 years) underwent second PCR. Among these, 161 (51.7%) were female. In total, 55 (17.6%) cases (mean age ± SD: 45.7 ± 18.1 years) were found positive for respiratory viruses panel in the second PCR. The HCoV-OC43/HKU1 was in 5.4% (17/311), Flu A in 4.5% (14/311), HCoV-229E/NL63 in 2.8% (9/311), HMPV in 1.9% (6/311), HPiV 1, 2, 3 in 1.2% (4/311), HRSV in 0.9% (3/311), and HAdV in 0.6% (2/311) of the cases studies. Also, co-infection was detected in 4 samples (1.2%). In addition, sore throat (0.028), headache (p = 0.016), and body pain (p = 0.0001) were statistically the most significant symptoms in studied cases.
UNASSIGNED: According to the findings of our study, respiratory virus infections and co-infections were 17.6% and 1.2% frequent, respectively. Interestingly, nearly half of our positive cases (47.2%) were identified by coronaviruses (ОС43, Е229, NL63, and HKUI), followed by influenza A virus (25.4%). However, for more comprehensive results, we recommend using greater sample size.
■在一项横断面研究中,使用HiTeq进行实时PCR。17病毒呼吸道病原体一步RT-PCR试剂盒(Genova,邦达·法拉瓦,Bioluence,德黑兰,伊朗)。
■共有311人(平均年龄±SD:48.2±21.7岁,范围:1-97年)进行了第二次PCR。其中,161名(51.7%)为女性。总的来说,在第二次PCR中发现55(17.6%)例(平均年龄±SD:45.7±18.1岁)呼吸道病毒阳性。HCoV-OC43/HKU1为5.4%(17/311),甲型流感占4.5%(14/311),HCoV-229E/NL63为2.8%(9/311),HMPV为1.9%(6/311),HPiV1、2、3的1.2%(4/311),HRSV为0.9%(3/311),和HAdV在0.6%(2/311)的病例研究中。此外,在4份样本中检测到共感染(1.2%)。此外,喉咙痛(0.028),头痛(p=0.016),和身体疼痛(p=0.0001)在统计学上是研究病例中最显著的症状。
■根据我们的研究结果,呼吸道病毒感染和合并感染的频率分别为17.6%和1.2%,分别。有趣的是,我们近一半的阳性病例(47.2%)是由冠状病毒鉴定的(43、229、NL63和HKUI),其次是甲型流感病毒(25.4%)。然而,为了获得更全面的结果,我们建议使用更大的样本量。