Abstract：
Antioxidant responses of juvenile sole exposed to seawater acidification (SA) and Cd were investigated. SA increased lipid peroxidation (LPO) in the fish, independent of Cd concentrations. Cd at medium and high levels inflated LPO under no or moderate SA conditions. This effect was absent under high SA levels, due to SA effect exceeding and obscuring Cd effect. SA and Cd collaborated to provoke LPO, with SOD and CAT being stimulated to defend against oxidative stress, while those related to GSH redox cycle were inhibited under SA exposure. Responses of GSH-related antioxidants to Cd impact varied contingent on their interactions with SA. This defensive strategy was insufficient to protect fish from increased LPO. Antioxidants responded more sensitively to SA than Cd exposure. GSH, GR, SOD and CAT are sensitive biomarkers for SA conditions. The findings offer insights into assessing fish\'s antioxidant defense strategy under Cd and SA circumstances in natural habitats.
摘要：
研究了暴露于海水酸化（SA）和Cd的幼体鞋底的抗氧化反应。SA增加了鱼类的脂质过氧化（LPO），与Cd浓度无关。在无或中等SA条件下，中等和高水平的Cd膨胀LPO。这种效应在高SA水平下不存在，由于SA效应超过和模糊Cd效应。SA和Cd合作挑起了LPO，SOD和CAT被刺激以防御氧化应激，而与GSH氧化还原循环相关的物质在SA暴露下受到抑制。GSH相关抗氧化剂对Cd影响的响应取决于它们与SA的相互作用。这种防御策略不足以保护鱼类免受LPO增加的影响。抗氧化剂对SA的反应比Cd暴露更敏感。GSH,GR,SOD和CAT是SA条件的敏感生物标志物。这些发现为评估自然栖息地中Cd和SA环境下鱼类的抗氧化防御策略提供了见解。
