Abstract：
Potential G-quadruplex forming sequences (PQS) are enriched in cancer-related genes and immunoglobulin class-switch recombination. They are prevalent in the 5\'UTR of transcriptionally active genes, thereby contributing to the regulation of gene expression. We and others previously demonstrated that the PQS located in the non-template strand leads to an R-loop formation followed by a G-quadruplex (G4) formation during transcription. These structural changes increase mRNA production. Here, we present how single-molecule technique was used to observe cotranscriptional G4 and R-loop formation and to examine the impact on transcription, particularly for the initiation and elongation stages.
摘要：
潜在的G-四链体形成序列（PQS）富含癌症相关基因和免疫球蛋白类别转换重组。它们在转录活性基因的5'UTR中普遍存在，从而有助于基因表达的调节。我们和其他人先前证明，位于非模板链中的PQS导致R环形成，然后在转录过程中形成G-四链体（G4）。这些结构变化增加了mRNA的产生。这里,我们介绍了如何使用单分子技术来观察共转录G4和R环的形成，并检查对转录的影响，特别是对于起始和延伸阶段。
