Abstract：
Nerve agents are the most notorious substances, which can be fatal to an individual because they block the activity of acetylcholinesterase. Fighting against unpredictable terrorist assaults and wars requires the simple and quick detection of chemical warfare agent vapor. In the present contribution, we have introduced a rhodamine-based chemosensor, BDHA, for the detection of nerve gas-mimicking agents diethylchlorophosphate (DCP) and diethylcyanophosphonate (DCNP) and mustard gas-mimicking agent 2-chloroethyl ethyl sulfide (CEES), both in the liquid and vapor phase. Probe BDHA provides the ability for detection by the naked eye in terms of colorimetric and fluorometric changes. It has been revealed that the interaction between nerve agents mimics and probe BDHA facilitates spirolactam ring opening due to the phosphorylation process. Thus, the highly fluorescent and colored species developed while probe BDHA is colorless and non-fluorescent due to the intramolecular spirolactam ring. Moreover, probe BDHA can effectively recognize DCP, DCNP, and CEES in the µM range despite many toxic analytes and could be identified based on the response times and quantum yield values. Inexpensive, easily carried paper strips-based test kits were developed for the quick, on-location solid and vapor phase detection of these mustard gas imitating agents (CEES) and nerve gas mimicking agents (DCP and DCNP) without needing expensive equipment or skilled personnel. More remarkably, the test strips\' color and fluorescence can be rapidly restored, exposing them to triethyl amine (TEA) for cyclic use, suggesting a potential application in the real-time identification of chemical warfare agents. To accomplish the on-location application of BDHA, we have experimented with soil samples to find traces of DCP. Therefore, the chromo-fluorogenic probe BDHA is a promising, instantaneous, and on-the-spot monitoring tool for the selective detection of DCP, DCNP, and CEES in the presence of others.
摘要：
神经毒剂是最臭名昭著的物质，这对个体来说可能是致命的，因为它们阻断了乙酰胆碱酯酶的活性。与不可预测的恐怖袭击和战争作斗争需要简单快速地检测化学战剂蒸气。在目前的贡献中，我们引入了一种基于罗丹明的化学传感器，BDHA,用于检测神经毒气模拟剂氯磷酸二乙酯（DCP）和氰基膦酸二乙酯（DCNP）和芥子气模拟剂2-氯乙基乙基硫醚（CEES），在液相和气相。探针BDHA提供了用肉眼检测比色和荧光变化的能力。已经揭示了神经剂模拟物和探针BDHA之间的相互作用由于磷酸化过程而促进螺内酰胺环打开。因此，由于分子内螺内酰胺环,探针BDHA是无色和非荧光的,因此开发出高度荧光和有色的物种。此外，探针BDHA能有效识别DCP,DCNP,尽管有许多有毒分析物，但CEES在µM范围内，可以根据响应时间和量子产率值进行识别。便宜,易于携带的基于纸条的测试套件被开发用于快速，这些芥子气模拟剂（CEES）和神经毒气模拟剂（DCP和DCNP）的现场固相和气相检测，无需昂贵的设备或技术人员。更值得注意的是,测试条的颜色和荧光可以迅速恢复，将它们暴露于三乙胺（TEA）以进行循环使用，提出了在实时识别化学战剂中的潜在应用。为了完成BDHA的现场应用，我们对土壤样本进行了实验，以发现DCP的痕迹。因此,色度荧光探针BDHA是一种很有前途的，瞬时，和现场监测工具，用于选择性检测DCP，DCNP,和CEES在别人面前。
