Abstract：
Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated under heat stress conditions. In contrast, pathways related to the cell cycle were suppressed under normal environmental temperatures. In line with the transcriptome analysis, the microbial analysis results indicate that taxonomic changes affect lipid degradation. Heat stress engendered statistically significant difference in the abundance of 11 microorganisms, including Bacteroides and Peptostreptococcacea. Together, integrative approach analysis suggests that microbiota-induced metabolites affect host fatty acid peroxidation metabolism, which is correlated with the gene families of Acyl-CoA dehydrogenase long chain (ACADL), Acyl-CoA Oxidase (ACOX) and Acetyl-CoA Acyltransferase (ACAA). This integrated approach provides novel insights into heat stress problems and identifies potential biomarkers associated with heat stress.
摘要：
尽管热应激对家畜动物有重大威胁，只有少数研究考虑了肉鸡与其微生物群之间的潜在关系。因此,这项研究检查了微生物修饰，使用基于代谢组数据的预测方法来了解热应激对家禽的影响。经过分析,宿主功能富集分析显示，在热应激条件下，与脂质和蛋白质代谢相关的途径升高。相比之下,与细胞周期相关的通路在正常环境温度下被抑制。根据转录组分析，微生物分析结果表明，分类学变化影响脂质降解。热应激导致11种微生物的丰度差异具有统计学意义，包括拟杆菌和肽链球菌。一起,综合方法分析表明，微生物群诱导的代谢产物影响宿主脂肪酸过氧化代谢，与酰基辅酶A脱氢酶长链（ACADL）的基因家族相关，酰基辅酶A氧化酶(ACOX)和乙酰辅酶A酰基转移酶(ACAA)。这种综合方法提供了对热应激问题的新见解，并确定了与热应激相关的潜在生物标志物。
