Abstract：
DNA-based methods are reliable for a precise identification of species in processed products. In this study, we assessed five typical DNA extraction methods from multiple aspects. Full-length and mini-length DNA barcoding were performed to detect the species substitution and mislabeling of 305 processed fish products from the Chinese market covering six processed fish products. The salt extraction method that exhibited the best overall performance was applied. All samples were successfully extracted; however, only 19.3 % of samples could be amplified using the full-DNA barcode primer set, and 90.2 % of samples could be amplified using the newly designed mini-DNA barcode primer sets (401 and 320 bp). Overall, the molecular identification results revealed that 36.4 % (111/305) of the samples were inconsistent with the labels, with commercial fraud observed in all six types of processed fish products. The survey findings provide technical references for effective fish authentication monitoring, offering insights into the seafood safety in markets.
摘要：
基于DNA的方法对于精确鉴定加工产品中的物种是可靠的。在这项研究中,我们从多个方面评估了五种典型的DNA提取方法。进行了全长和微型DNA条形码编码，以检测来自中国市场的305种加工鱼产品的物种替代和错误标记，涵盖了6种加工鱼产品。采用表现出最佳整体性能的盐提取方法。所有样品均已成功提取；然而，只有19.3%的样品可以使用全DNA条形码引物组扩增，使用新设计的微型DNA条形码引物组（401和320bp）可以扩增90.2%的样品。总的来说,分子鉴定结果表明，36.4%(111/305)的样品与标签不一致，在所有六种加工鱼产品中观察到商业欺诈。调查结果为有效的鱼类认证监测提供了技术参考,提供对海鲜市场安全的见解。
