Abstract：
Hesperidin is a phenolic compound usually found in citrus fruits, which is known for its anti-inflammatory and antioxidant properties. This bioactive compound has already been used to formulate medications to treat chronic venous insufficiency. In this work, through a system which allows the in-line coupling of the pressurized liquid extraction (PLE) and high-intensity ultrasound (HIUS) with solid phase extraction (SPE), and analysis by high-performance liquid chromatography with UV-Vis detector (HPLC-UV) in on-line mode, a method was developed to obtain, separate, and quantify hesperidin from the industrial waste of lime. An eco-friendly approach with water and ethanol as extraction solvents was used. Parameters such as temperature (80, 100, and 120 °C) and HIUS power (0, 200, and 400 W) were evaluated regarding hesperidin yield. In this context, the higher hesperidin yield (18.25 ± 1.52 mg/g) was achieved using water at a subcritical state (120 °C and 15 MPa). The adsorbent SepraTM C-18-E isolated hesperidin from the other extracted compounds employing 50% ethanol in the SPE elution. The possibility ofon-lineanalysis coupling a high-performance liquid chromatograph to an ultraviolet detector (HPLC-UV) system was studied and shown to be a feasible approach for developing integrated technologies. Conventional extractions and their antioxidant capacities were evaluated, highlighting the advantages of the HIUS-PLE-SPE extractive method. Furthermore, the on-linechromatographic analysis showed the potential of the HIUS-PLE-SPE- HPLC-UV system to quantify the extracted compounds in real time.
摘要：
橙皮苷是通常在柑橘类水果中发现的酚类化合物，以其抗炎和抗氧化特性而闻名。这种生物活性化合物已经用于配制治疗慢性静脉功能不全的药物。在这项工作中,通过一个系统，该系统允许加压液体萃取（PLE）和高强度超声（HIUS）与固相萃取（SPE）的在线耦合，并通过高效液相色谱法与紫外-可见检测器（HPLC-UV）在在线模式下进行分析，开发了一种方法来获得，分开,并从石灰的工业废料中量化橙皮苷。使用水和乙醇作为提取溶剂的生态友好方法。关于橙皮苷产率评估诸如温度(80、100和120°C)和HIUS功率(0、200和400W)的参数。在这种情况下，使用亚临界状态（120°C和15MPa）的水可以获得更高的橙皮苷产量（18.25±1.52mg/g）。吸附剂SepraTMC-18-E在SPE洗脱中使用50％乙醇从其他提取的化合物中分离出橙皮苷。研究了在线分析将高效液相色谱仪耦合到紫外检测器（HPLC-UV）系统的可能性，并证明这是开发集成技术的可行方法。常规提取及其抗氧化能力进行了评估，突出了HIUS-PLE-SPE萃取方法的优点。此外，在线色谱分析显示了HIUS-PLE-SPE-HPLC-UV系统实时定量提取的化合物的潜力。
