Abstract：
Extracranial waste transport from the brain interstitial fluid to the deep cervical lymph node (dCLN) is not extensively understood. The present study aims to show the cranial nerves that have a role in the transport of brain lymphatics vessels (LVs), their localization, diameter, and number using podoplanin (PDPN) and CD31 immunohistochemistry (IHC) and Western blotting. Cranial nerve samples from 6 human cases (3 cadavers, and 3 autopsies) were evaluated for IHC and 3 autopsies for Western blotting. The IHC staining showed LVs along the optic, olfactory, oculomotor, trigeminal, facial, glossopharyngeal, accessory, and vagus nerves. However, no LVs present along the trochlear, abducens, vestibulocochlear, and hypoglossal nerves. The LVs were predominantly localized at the endoneurium of the cranial nerve that has motor components, and LVs in the cranial nerves that had sensory components were present in all 3 layers. The number of LVs accompanying the olfactory, optic, and trigeminal nerves was classified as numerous; oculomotor, glossopharyngeal, vagus, and accessory was moderate; and facial nerves was few. The largest diameter of LVs was in the epineurium and the smallest one was in the endoneurium. The majority of Western blotting results correlated with the IHC. The present findings suggest that specific cranial nerves with variable quantities provide a pathway for the transport of wastes from the brain to dCLN. Thus, the knowledge of the transport of brain lymphatics along cranial nerves may help understand the pathophysiology of various neurological diseases.
摘要：
颅外废物从脑间质液到颈深淋巴结（dCLN）的运输尚未得到广泛了解。本研究旨在显示在脑淋巴管（LV）运输中起作用的颅神经，他们的本地化，直径,使用足planin（PDPN）和CD31免疫组织化学（IHC）和蛋白质印迹法进行计数。来自6例人类病例的颅神经样本（3具尸体，和3次尸检）进行了IHC评估，3次尸检进行了Western印迹评估。IHC染色显示沿着视神经的LV，嗅觉,动眼,三叉神经,面部,舌咽,附件,和迷走神经.然而,滑车上没有LV，绑架者,前庭耳蜗,和舌下神经.LV主要位于具有运动成分的颅神经内膜，在所有3层中都存在具有感觉成分的颅神经中的LV。伴随嗅觉的LV的数量，光学的,三叉神经被分类为许多；动眼神经，舌咽,迷走神经,附件适中；面神经很少。LV的最大直径在神经外膜中，最小的直径在神经内膜中。大多数Western印迹结果与IHC相关。目前的发现表明，具有可变数量的特定颅神经为废物从大脑到dCLN的运输提供了途径。因此，了解脑淋巴管沿着颅神经的运输可能有助于了解各种神经系统疾病的病理生理。
