Abstract：
Electrochemically active bacteria (EAB) exhibit promising prospects for space exploration and life support systems. However, the effects of the space environment on EAB are unclear. In this study, the effects of simulated microgravity on the current generation of mixed-culture EAB were illustrated, and the underlying mechanism was elucidated. The results demonstrated that the electrochemical activity of mixed-culture EAB was enhanced, which was mainly due to the enrichment of Geobacter and the increase in EAB biomass. Additionally, the genes and proteins of the biofilm changed obviously under simulated microgravity conditions, including: I) genes related to signal transfer, II) genes related to cell wall synthesis, and III) genes related to riboflavin synthesis. This study first revealed the enrichment in EAB abundance, the increase in EAB biomass, and the promotion of current generation under simulated microgravity.
摘要：
电化学活性细菌（EAB）在太空探索和生命支持系统中具有广阔的前景。然而,空间环境对EAB的影响尚不清楚。在这项研究中,说明了模拟微重力对当前一代混合培养EAB的影响，并阐明了潜在的机制。结果表明，混合培养EAB的电化学活性得到增强，这主要是由于Geobacter的富集和EAB生物量的增加。此外,在模拟微重力条件下，生物膜的基因和蛋白质发生了明显的变化，包括：I)与信号传递相关的基因，II）与细胞壁合成相关的基因，和III)与核黄素合成相关的基因。这项研究首次揭示了EAB丰度的富集，EAB生物量的增加，以及在模拟微重力下促进电流产生。
