PDF(Pubmed)
Abstract：
Inhibiting oxidative stress is key for ensuring sperm motility during semen cryopreservation. The aim of this study was to investigate the effect of adding alpha-lipoic acid (ALA) as an extender in rooster semen cryopreservation. Different concentrations of ALA were added to the frozen diluent of rooster semen; subsequently, computer-aided semen analysis was used to determine membrane functional integrity, acrosome integrity, antioxidant capacity (based on T-AOC, GSH-Px, SOD, CAT, and MDA contents), and mitochondrial integrity. The frozen sperm ultrastructure was observed using transmission electron microscopy. The results showed that the addition of different concentrations of ALA partially to greatly improved the quality of frozen sperm; in particular, 8 μg/mL ALA significantly improved multiple parameters of sperm quality, including sperm motility and antioxidant enzyme activity, after freeze-thaw. The results of this study provide empirical and theoretical support for effective rooster semen cryopreservation and can inform the development of new protective agents in the field of livestock reproduction.
摘要：
抑制氧化应激是确保精液冷冻保存过程中精子活力的关键。本研究的目的是研究添加α-硫辛酸（ALA）作为补充剂在公鸡精液冷冻保存中的作用。鸡精液冷冻稀释液中加入不同浓度的ALA,计算机辅助精液分析用于确定膜功能完整性，顶体完整性,抗氧化能力(基于T-AOC，GSH-Px,SOD,CAT,和MDA含量)，和线粒体的完整性。采用透射电镜观察冷冻精子超微结构。结果表明,添加不同浓度的ALA部分可以大大提高冷冻精子的质量,8μg/mLALA能显著提高精子质量的多项指标,包括精子活力和抗氧化酶活性，冻融后。本研究结果为有效的公鸡精液冷冻保存提供了经验和理论支持，可为家畜繁殖领域新型保护剂的开发提供参考。
