Abstract：
The TRIM family is associated with the membrane, and its involvement in the progression, growth, and development of various cancer types has been researched extensively. However, the role played by the TRIM5 gene within this family has yet to be explored to a great extent in terms of hepatocellular carcinoma (HCC). The data of patients relating to mRNA expression and the survival rate of individuals diagnosed with HCC were extracted from The Cancer Genome Atlas (TCGA) database. UALCAN was employed to examine the potential link between TRIM5 expression and clinicopathological characteristics. In addition, enrichment analysis of differentially expressed genes (DEGs) was conducted as a means of deciphering the function and mechanism of TRIM5 in HCC. The data in the TCGA and TIMER2.0 databases was utilized to explore the correlation between TRIM5 and immune infiltration in HCC. WGCNA was performed as a means of assessing TRIM5-related co-expressed genes. The \"OncoPredict\" R package was also used for investigating the association between TRIM5 and drug sensitivity. Finally, qRT-PCR, Western blotting (WB) and immunohistochemistry (IHC) were employed for exploring the differential expression of TRIM5 and its clinical relevance in HCC. According to the results that were obtained from the vitro experiments, mRNA and protein levels of TRIM5 demonstrated a significant upregulation in HCC tissues. It is notable that TRIM5 expression levels were found to have a strong association with the infiltration of diverse immune cells and displayed a positive correlation with several immune checkpoint inhibitors. The TRIM5 expression also displayed promising clinical prognostic value for HCC patients.
摘要：
TRIM家族与膜相关，以及它在进步中的参与，增长,和各种癌症类型的发展已经被广泛研究。然而,在肝细胞癌(HCC)方面,TRIM5基因在该家族中所起的作用尚未得到很大程度的探索.从癌症基因组图谱（TCGA）数据库中提取与mRNA表达相关的患者数据和诊断为HCC的个体的存活率。UALCAN用于检查TRIM5表达与临床病理特征之间的潜在联系。此外,进行了差异表达基因（DEGs）的富集分析，以破译TRIM5在HCC中的功能和机制。TCGA和TIMER2.0数据库中的数据用于探索TRIM5与HCC中免疫浸润之间的相关性。进行WGCNA作为评估TRIM5相关共表达基因的手段。“OncoPredict”R软件包也用于研究TRIM5与药物敏感性之间的关联。最后,qRT-PCR,蛋白质印迹（WB）和免疫组织化学（IHC）用于探讨TRIM5的差异表达及其在HCC中的临床意义。根据从体外实验中获得的结果，TRIM5的mRNA和蛋白质水平在HCC组织中显示出显着的上调。值得注意的是,发现TRIM5表达水平与多种免疫细胞的浸润具有强相关性,并且显示与几种免疫检查点抑制剂的正相关性。TRIM5表达对HCC患者也显示出有希望的临床预后价值。
