PDF(Pubmed)
Abstract：
Hippoboscid flies are bloodsucking arthropods that can transmit pathogenic microorganisms and are therefore potential vectors for pathogens such as Bartonella spp. These Gram-negative bacteria can cause mild-to-severe clinical signs in humans and animals; therefore, monitoring Bartonella spp. prevalence in louse fly populations appears to be a useful prerequisite for zoonotic risk assessment.
Using convenience sampling, we collected 103 adult louse flies from four ked species (Lipoptena cervi, n = 22; Lipoptena fortisetosa, n = 61; Melophagus ovinus, n = 12; Hippobosca equina, n = 8) and the pupae of M. ovinus (n = 10) in the federal state of Saxony, Germany. All the samples were screened by polymerase chain reaction (PCR) for Bartonella spp. DNA, targeting the citrate synthase gene (gltA). Subsequently, PCRs targeting five more genes (16S, ftsZ, nuoG, ribC and rpoB) were performed for representatives of revealed gltA genotypes, and all the PCR products were sequenced to identify the Bartonella (sub)species accurately.
The overall detection rates for Bartonella spp. were 100.0%, 59.1%, 24.6% and 75.0% in M. ovinus, L. cervi, L. fortisetosa and H. equina, respectively. All the identified bartonellae belong to the Bartonella schoenbuchensis complex. Our data support the proposed reclassification of the (sub)species status of this group, and thus we conclude that several genotypes of B. schoenbuchensis were detected, including Bartonella schoenbuchensis subsp. melophagi and Bartonella schoenbuchensis subsp. schoenbuchensis, both of which have previously validated zoonotic potential. The extensive PCR analysis revealed the necessity of multiple PCR approach for proper identification of the ruminant-associated bartonellae.
Using convenience sampling, we collected 103 adult louse flies from four ked species (Lipoptena cervi, n = 22; Lipoptena fortisetosa, n = 61; Melophagus ovinus, n = 12; Hippobosca equina, n = 8) and the pupae of M. ovinus (n = 10) in the federal state of Saxony, Germany. All the samples were screened by polymerase chain reaction (PCR) for Bartonella spp. DNA, targeting the citrate synthase gene (gltA). Subsequently, PCRs targeting five more genes (16S, ftsZ, nuoG, ribC and rpoB) were performed for representatives of revealed gltA genotypes, and all the PCR products were sequenced to identify the Bartonella (sub)species accurately.
The overall detection rates for Bartonella spp. were 100.0%, 59.1%, 24.6% and 75.0% in M. ovinus, L. cervi, L. fortisetosa and H. equina, respectively. All the identified bartonellae belong to the Bartonella schoenbuchensis complex. Our data support the proposed reclassification of the (sub)species status of this group, and thus we conclude that several genotypes of B. schoenbuchensis were detected, including Bartonella schoenbuchensis subsp. melophagi and Bartonella schoenbuchensis subsp. schoenbuchensis, both of which have previously validated zoonotic potential. The extensive PCR analysis revealed the necessity of multiple PCR approach for proper identification of the ruminant-associated bartonellae.
摘要：
背景：河马果蝇是吸血节肢动物，可以传播病原微生物，因此是病原体如巴尔通体的潜在载体。这些革兰氏阴性菌可在人类和动物中引起轻度至重度的临床体征；因此，监测巴尔通菌属。虱虫种群的患病率似乎是人畜共患风险评估的有用前提。
方法：使用方便的抽样，我们从四个ked物种（Lipoptenacervi，n=22;fortisetosalipoptenafortisetosa,n=61；龙舌兰，n=12；马匹，n=8）和萨克森州联邦州的M.ovinus（n=10）的p，德国。通过聚合酶链反应（PCR）筛选所有样品中的巴尔通菌。DNA,靶向柠檬酸合酶基因（gltA）。随后，靶向五个以上基因的PCRs(16S，ftsZ,nuoG,对揭示的gltA基因型的代表进行了ribC和rpoB)，并对所有PCR产物进行测序，以准确鉴定巴尔通体（亚）种。
结论：巴尔通体的总体检出率。是100.0%，59.1%,M.ovinus中的24.6%和75.0%，L.cervi,L.fortisetosa和马匹，分别。所有已鉴定的Bartonellae都属于Bartonellaschanenbuchensis复合体。我们的数据支持对该组（亚）种状态的重新分类，因此，我们得出结论，B.schoenbuchensis的几个基因型被检测到，包括巴尔通菌亚种。墨罗哈木和巴尔通菌亚种。schoenbuchensis,两者先前都验证了人畜共患的潜力。广泛的PCR分析显示，必须采用多种PCR方法来正确鉴定反刍动物相关的bartonellae。
方法：使用方便的抽样，我们从四个ked物种（Lipoptenacervi，n=22;fortisetosalipoptenafortisetosa,n=61；龙舌兰，n=12；马匹，n=8）和萨克森州联邦州的M.ovinus（n=10）的p，德国。通过聚合酶链反应（PCR）筛选所有样品中的巴尔通菌。DNA,靶向柠檬酸合酶基因（gltA）。随后，靶向五个以上基因的PCRs(16S，ftsZ,nuoG,对揭示的gltA基因型的代表进行了ribC和rpoB)，并对所有PCR产物进行测序，以准确鉴定巴尔通体（亚）种。
结论：巴尔通体的总体检出率。是100.0%，59.1%,M.ovinus中的24.6%和75.0%，L.cervi,L.fortisetosa和马匹，分别。所有已鉴定的Bartonellae都属于Bartonellaschanenbuchensis复合体。我们的数据支持对该组（亚）种状态的重新分类，因此，我们得出结论，B.schoenbuchensis的几个基因型被检测到，包括巴尔通菌亚种。墨罗哈木和巴尔通菌亚种。schoenbuchensis,两者先前都验证了人畜共患的潜力。广泛的PCR分析显示，必须采用多种PCR方法来正确鉴定反刍动物相关的bartonellae。
