PDF(Pubmed)
Abstract：
The advancement of genetic code expansion (GCE) technology is attributed to the establishment of specific aminoacyl-tRNA synthetase/tRNA pairs. While earlier improvements mainly focused on aminoacyl-tRNA synthetases, recent studies have highlighted the importance of optimizing tRNA sequences to enhance both unnatural amino acid incorporation efficiency and orthogonality. Given the crucial role of tRNAs in the translation process and their substantial impact on overall GCE efficiency, ongoing efforts are dedicated to the development of tRNA engineering techniques. This review explores diverse tRNA engineering approaches and provides illustrative examples in the context of GCE, offering insights into the user-friendly implementation of GCE technology.
摘要：
遗传密码扩展（GCE）技术的进步归因于特异性氨酰基-tRNA合成酶/tRNA对的建立。虽然早期的改进主要集中在氨酰基-tRNA合成酶上，最近的研究强调了优化tRNA序列以提高非天然氨基酸掺入效率和正交性的重要性。鉴于tRNA在翻译过程中的关键作用及其对整体GCE效率的重大影响，正在进行的努力致力于tRNA工程技术的发展。这篇综述探讨了不同的tRNA工程方法，并在GCE的背景下提供了说明性的例子，提供对GCE技术的用户友好实施的见解。
