PDF(Pubmed)
Abstract：
Rapeseed is a significant global source of plant oil. Silique size, particularly silique length (SL), impacts rapeseed yield. SL is a typical quantitative trait controlled by multiple genes. In our previous study, we constructed a DH population of 178 families known as the 158A-SGDH population. In this study, through SL QTL mapping, we identified twenty-six QTL for SL across five replicates in two environments. A QTL meta-analysis revealed eight consensus QTL, including two major QTL: cqSL.A02-1 (11.32-16.44% of PVE for SL), and cqSL.C06-1 (10.90-11.95% of PVE for SL). Based on biparental resequencing data and microcollinearity analysis of target regions in Brassica napus and Arabidopsis, we identified 11 candidate genes at cqSL.A02-1 and 6 candidate genes at cqSL.C06-1, which are potentially associated with silique development. Furthermore, transcriptome analysis of silique valves from both parents on the 14th, 21st, and 28th days after pollination (DAP) combined with gene function annotation revealed three significantly differentially expressed genes at cqSL.A02-1, BnaA02G0058500ZS, BnaA02G0060100ZS, and BnaA02G0060900ZS. Only the gene BnaC06G0283800ZS showed significant differences in parental transcription at cqSL.C06-1. Two tightly linked insertion-deletion markers for the cqSL.A02-1 and cqSL.C06-1 loci were developed. Using these two QTL, we generated four combinations: A02SGDH284C06158A, A02SGDH284C06SGDH284, A02158AC06158A, and A02158AC06SGDH284. Subsequent analysis identified an ideal QTL combination, A02158AC06SGDH284, which exhibited the longest SL of this type, reaching 6.06 ± 0.10 cm, significantly surpassing the other three combinations. The results will provide the basis for the cloning of SL-related genes of rapeseed, along with the development of functional markers of target genes and the breeding of rapeseed varieties.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01464-x.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01464-x.
摘要：
油菜籽是全球重要的植物油来源。Silique尺寸,特别是斜角长度(SL)，影响油菜籽产量。SL是由多基因控制的典型数量性状。在我们之前的研究中,我们构建了一个由178个家庭组成的DH种群，称为158A-SGDH种群。在这项研究中,通过SLQTL映射，我们在两个环境中的五个重复中确定了SL的26个QTL。QTL荟萃分析揭示了八个共有QTL，包括两个主要的QTL：cqSL。A02-1（用于SL的PVE的11.32-16.44%），和cqSL。C06-1（SL的PVE的10.90-11.95%）。基于甘蓝型油菜和拟南芥靶区域的双亲重测序数据和微共线性分析，我们在cqSL鉴定了11个候选基因。在cqSL的A02-1和6个候选基因。C06-1，这可能与角力发展有关。此外，14日对双亲角瓣的转录组分析，21日,授粉后第28天（DAP）结合基因功能注释揭示了三个在cqSL显着差异表达的基因。A02-1,BnaA02G0058500ZS,BnaA02G0060100ZS,和BnaA02G0060900ZS。只有基因BnaC06G0283800ZS在cqSL的亲本转录中显示出显著差异。C06-1.cqSL的两个紧密链接的插入-缺失标记。A02-1和cqSL。开发了C06-1基因座。利用这两个QTL，我们生成了四个组合：A02SGDH284C06158A，A02SGDH284C06SGDH284,A02158AC06158A,和A02158AC06SGDH284。随后的分析确定了理想的QTL组合，A02158AC06SGDH284，表现出这种类型中最长的SL，达到6.06±0.10厘米，大大超过其他三种组合。研究结果将为油菜SL相关基因的克隆提供依据,随着目标基因功能标记的发展和油菜品种的选育。
■在线版本包含补充材料，可在10.1007/s11032-024-01464-x获得。
■在线版本包含补充材料，可在10.1007/s11032-024-01464-x获得。
