METHODS: We conducted a comprehensive literature search in multiple databases, identifying eligible studies focusing on neurosurgical applications of endovascular biopsy. The systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The relevant information was collected, including study characteristics, biopsy techniques, and key findings.
RESULTS: Nine studies met the inclusion criteria and were included. The studies involved the collection of ECs using various endovascular devices including coils, guide wires, different stents, and forceps. Endothelial-enrichment techniques, such fluorescence-activated cell sorting (FACS), collected ECs and facilitated downstream applications of bulk or single-cell RNA sequencing (scRNAseq). The studies provided insights into gene expression profiles and identified potential biomarkers associated with intracranial aneurysms. However, challenges were observed in obtaining an adequate number of ECs and identifying consistent biomarkers.
CONCLUSIONS: Endovascular biopsy of endothelial cells (ECs) in cerebrovascular pathologies shows promise for gene expression profiling. However, many studies have been limited in sample size and underpowered to identify \"signature genes\" for aneurysm growth or rupture. Advancements in minimally invasive biopsy methods have potential to facilitate applications of precision medicine in the treatment of cerebrovascular disorders.
方法:我们在多个数据库中进行了全面的文献检索,确定符合条件的研究,重点是血管内活检的神经外科应用。系统审查遵循系统审查和荟萃分析(PRISMA)声明的首选报告项目。收集了相关信息,包括学习特点,活检技术,和关键发现。
结果:9项研究符合纳入标准并被纳入。研究涉及使用包括线圈在内的各种血管内装置收集ECs,导丝,不同的支架,和镊子.内皮富集技术,这种荧光激活细胞分选(FACS),收集EC并促进批量或单细胞RNA测序(scRNAseq)的下游应用。这些研究提供了对基因表达谱的见解,并确定了与颅内动脉瘤相关的潜在生物标志物。然而,在获得足够数量的内皮细胞和确定一致的生物标志物方面观察到挑战.
结论:脑血管疾病中内皮细胞(ECs)的血管内活检显示了基因表达谱的前景。然而,许多研究的样本量有限,在确定动脉瘤生长或破裂的“特征基因”方面的能力不足。微创活检方法的进步有可能促进精准医学在脑血管疾病治疗中的应用。