Abstract：
OBJECTIVE: This study aims to evaluate the impact of asiaticoside (AC) on the viability and proliferation of dental pulp stem cells (DPSCs), considering the known negative effects of routinely used intracanal medicaments. This evaluation will be compared with the outcomes from using traditional intracanal medicaments, specifically triple antibiotic paste (TAP) and calcium hydroxide [Ca(OH)2].
METHODS: The DPSCs were obtained from the third molars of an adult donor. The application of flow cytometry was employed to do a phenotypic analysis on DPSCs using CD90, CD73, CD105, CD34, CD14, and CD45 antibodies. The methylthiazol tetrazolium (MTT) assay was employed to assess cellular viability. The cells were treated with different concentrations of TAP and Ca(OH)2 (5, 2.5, 1, 0.5, and 0.25 mg/mL), along with AC (100, 50, 25, 12.5, and 6.25 µM). A cell proliferation rate was performed at 3, 5, and 7 days.
RESULTS: The characterization of DPSCs was conducted by flow cytometry analysis, which verified the presence of mesenchymal cell surface antigen molecules (CD105, CD73, and CD90) and demonstrated the absence of hematopoietic markers (CD34, CD45, and CD14). Cells treated with concentrations over 0.5 mg/mL of TAP and Ca(OH)2 showed a notable reduction in cell viability in comparison to the untreated cells (p < 0.05). Additionally, the cells treated with different concentrations of AC 12.5, 6.25, 25, and 50 µM did not differ significantly from the untreated cells (p > 0.05). Nevertheless, cells treated with concentrations of 100 µM showed a significant reduction in viability compared to the untreated cells (p < 0.05). After a period of 7 days, it was noted that cells exposed to three different concentrations of AC (50, 25, and 12.5 µM) had a notable rise in cell density in comparison to TAP and Ca(OH)2 (p < 0.05). Furthermore, cells that were exposed to a concentration of 12.5 µM exhibited the highest cell density.
CONCLUSIONS: The cellular viability of the AC-treated cells was superior to that of the TAP and Ca(OH)2-treated cells. Moreover, the AC with a concentration of 12.5 µM had the highest degree of proliferation.
CONCLUSIONS: This study underscores the importance of evaluating alternative root canal medicaments and their effects on DPSCs\' growth and vitality. The findings on AC, particularly its influence on the survival and proliferation of DPSCs, offer valuable insights for its probable use as an intracanal medication. This research contributes to the ongoing efforts to identify safer and more effective intracanal treatments, which are crucial for enhancing patient outcomes in endodontic procedures. How to cite this article: Alazemi MJ, Badawi MF, Elbeltagy MG, et al. Examining the Effects of Asiaticoside on Dental Pulp Stem Cell Viability and Proliferation: A Promising Approach to Root Canal Treatment. J Contemp Dent Pract 2024;25(2):118-127.
METHODS: The DPSCs were obtained from the third molars of an adult donor. The application of flow cytometry was employed to do a phenotypic analysis on DPSCs using CD90, CD73, CD105, CD34, CD14, and CD45 antibodies. The methylthiazol tetrazolium (MTT) assay was employed to assess cellular viability. The cells were treated with different concentrations of TAP and Ca(OH)2 (5, 2.5, 1, 0.5, and 0.25 mg/mL), along with AC (100, 50, 25, 12.5, and 6.25 µM). A cell proliferation rate was performed at 3, 5, and 7 days.
RESULTS: The characterization of DPSCs was conducted by flow cytometry analysis, which verified the presence of mesenchymal cell surface antigen molecules (CD105, CD73, and CD90) and demonstrated the absence of hematopoietic markers (CD34, CD45, and CD14). Cells treated with concentrations over 0.5 mg/mL of TAP and Ca(OH)2 showed a notable reduction in cell viability in comparison to the untreated cells (p < 0.05). Additionally, the cells treated with different concentrations of AC 12.5, 6.25, 25, and 50 µM did not differ significantly from the untreated cells (p > 0.05). Nevertheless, cells treated with concentrations of 100 µM showed a significant reduction in viability compared to the untreated cells (p < 0.05). After a period of 7 days, it was noted that cells exposed to three different concentrations of AC (50, 25, and 12.5 µM) had a notable rise in cell density in comparison to TAP and Ca(OH)2 (p < 0.05). Furthermore, cells that were exposed to a concentration of 12.5 µM exhibited the highest cell density.
CONCLUSIONS: The cellular viability of the AC-treated cells was superior to that of the TAP and Ca(OH)2-treated cells. Moreover, the AC with a concentration of 12.5 µM had the highest degree of proliferation.
CONCLUSIONS: This study underscores the importance of evaluating alternative root canal medicaments and their effects on DPSCs\' growth and vitality. The findings on AC, particularly its influence on the survival and proliferation of DPSCs, offer valuable insights for its probable use as an intracanal medication. This research contributes to the ongoing efforts to identify safer and more effective intracanal treatments, which are crucial for enhancing patient outcomes in endodontic procedures. How to cite this article: Alazemi MJ, Badawi MF, Elbeltagy MG, et al. Examining the Effects of Asiaticoside on Dental Pulp Stem Cell Viability and Proliferation: A Promising Approach to Root Canal Treatment. J Contemp Dent Pract 2024;25(2):118-127.
摘要：
目的：本研究旨在评估积雪草苷（AC）对牙髓干细胞（DPSC）的活力和增殖的影响，考虑到常规使用的肛门内药物的已知负面影响。此评估将与使用传统肛门内药物的结果进行比较，特别是三重抗生素糊（TAP）和氢氧化钙[Ca（OH）2]。
方法:从成年供体的第三磨牙获得DPSC。流式细胞术的应用用于使用CD90、CD73、CD105、CD34、CD14和CD45抗体对DPSC进行表型分析。使用甲基噻唑四唑(MTT)测定来评估细胞活力。用不同浓度的TAP和Ca（OH）2（5、2.5、1、0.5和0.25mg/mL）处理细胞，以及AC(100、50、25、12.5和6.25µM)。在第3、5和7天进行细胞增殖率。
结果：通过流式细胞术分析对DPSC进行表征，这证实了间充质细胞表面抗原分子（CD105，CD73和CD90）的存在，并证明了造血标志物（CD34，CD45和CD14）的缺乏。与未处理的细胞相比,用浓度超过0.5mg/mL的TAP和Ca(OH)2处理的细胞显示细胞活力显著降低(p<0.05)。此外,用不同浓度的AC12.5,6.25,25和50µM处理的细胞与未处理的细胞没有显著差异(p>0.05).然而，与未处理的细胞相比，用100μM浓度处理的细胞显示出活力的显著降低(p<0.05)。经过7天的时间，值得注意的是,与TAP和Ca(OH)2相比,暴露于三种不同浓度AC(50,25和12.5µM)的细胞密度显著升高(p<0.05).此外，暴露于12.5μM浓度的细胞表现出最高的细胞密度.
结论:AC处理的细胞的细胞活力优于TAP和Ca(OH)2处理的细胞。此外，浓度为12.5µM的AC具有最高的增殖程度。
结论：本研究强调了评估替代根管药物及其对DPSC生长和活力的影响的重要性。关于AC的发现，特别是它对DPSC的存活和增殖的影响，为其可能用作肛门内药物提供有价值的见解。这项研究有助于正在进行的努力，以确定更安全，更有效的肛门内治疗，这对于提高牙髓手术的患者预后至关重要。如何引用这篇文章:AlazemiMJ,巴达维MF,ElbeltagyMG,etal.研究积雪草苷对牙髓干细胞活力和增殖的影响：根管治疗的有希望的方法。JContempDentPract2024；25(2)：118-127。
方法:从成年供体的第三磨牙获得DPSC。流式细胞术的应用用于使用CD90、CD73、CD105、CD34、CD14和CD45抗体对DPSC进行表型分析。使用甲基噻唑四唑(MTT)测定来评估细胞活力。用不同浓度的TAP和Ca（OH）2（5、2.5、1、0.5和0.25mg/mL）处理细胞，以及AC(100、50、25、12.5和6.25µM)。在第3、5和7天进行细胞增殖率。
结果：通过流式细胞术分析对DPSC进行表征，这证实了间充质细胞表面抗原分子（CD105，CD73和CD90）的存在，并证明了造血标志物（CD34，CD45和CD14）的缺乏。与未处理的细胞相比,用浓度超过0.5mg/mL的TAP和Ca(OH)2处理的细胞显示细胞活力显著降低(p<0.05)。此外,用不同浓度的AC12.5,6.25,25和50µM处理的细胞与未处理的细胞没有显著差异(p>0.05).然而，与未处理的细胞相比，用100μM浓度处理的细胞显示出活力的显著降低(p<0.05)。经过7天的时间，值得注意的是,与TAP和Ca(OH)2相比,暴露于三种不同浓度AC(50,25和12.5µM)的细胞密度显著升高(p<0.05).此外，暴露于12.5μM浓度的细胞表现出最高的细胞密度.
结论:AC处理的细胞的细胞活力优于TAP和Ca(OH)2处理的细胞。此外，浓度为12.5µM的AC具有最高的增殖程度。
结论：本研究强调了评估替代根管药物及其对DPSC生长和活力的影响的重要性。关于AC的发现，特别是它对DPSC的存活和增殖的影响，为其可能用作肛门内药物提供有价值的见解。这项研究有助于正在进行的努力，以确定更安全，更有效的肛门内治疗，这对于提高牙髓手术的患者预后至关重要。如何引用这篇文章:AlazemiMJ,巴达维MF,ElbeltagyMG,etal.研究积雪草苷对牙髓干细胞活力和增殖的影响：根管治疗的有希望的方法。JContempDentPract2024；25(2)：118-127。
