METHODS: Duplex allele-specific polymerase chain reaction (PCR) with an internal control was performed for HLA-B*59:01 genotyping. The accuracy of duplex allele-specific PCR for HLA-B*59:01 genotyping was evaluated in 200 blood samples, using sequence-based typing (SBT) as the reference method.
RESULTS: In total, 50 HLA-B*59:01-positive and 150 HLA-B*59:01-negative results obtained using duplex allele-specific PCR were in complete agreement with the SBT results.
CONCLUSIONS: Duplex allele-specific PCR is a rapid, reliable, and economical assay for screening the HLA-B*59:01 allele.
方法:用内部对照进行双重等位基因特异性聚合酶链反应(PCR)进行HLA-B*59:01基因分型。在200份血液样本中评估了双重等位基因特异性PCR对HLA-B*59:01基因分型的准确性,使用基于序列的分型(SBT)作为参考方法。
结果:总计,使用双链体等位基因特异性PCR获得的50个HLA-B*59:01阳性和150个HLA-B*59:01阴性结果与SBT结果完全一致。
结论:双重等位基因特异性PCR是一种快速,可靠,和筛选HLA-B*59:01等位基因的经济试验。