Abstract：
The bioactive activity of proanthocyanidins (PAs) is closely associated with their degree of polymerization (DP), however, the effects of PAs with different DP on digestion and gut microbiota have remained unclear. To investigate this, we conducted in vitro simulated digestion and colonic fermentation studies on samples of PAs with different DP. The results showed that PAs was influenced by both protein precipitation and enzymolysis, resulting in a decrease in functional activity. PAs with a high DP were more sensitive to the gastrointestinal environment. The significant clustering trend in colonic fermentation verified the reliability of multivariate statistical techniques for screening samples with distinct functional differences. The gut microbiota analysis showed that oligomeric PAs had a stronger promoting effect on beneficial bacteria, while high polymeric PAs had a greater inhibitory effect on harmful bacteria. This study offers new insights into the biological activity and microbiological mechanisms of PAs with different DP.
摘要：
原花青素(PAs)的生物活性与其聚合度(DP)密切相关,然而,不同DP的PAs对消化和肠道菌群的影响尚不清楚。为了调查这一点，我们对不同DP的PAs样品进行了体外模拟消化和结肠发酵研究。结果表明，蛋白质沉淀和酶解都会影响PAs，导致功能活动减少。具有高DP的PA对胃肠道环境更敏感。结肠发酵的显着聚类趋势验证了多元统计技术筛选具有明显功能差异的样品的可靠性。肠道菌群分析表明,寡聚PAs对有益菌有较强的促进作用,而高聚合物PAs对有害细菌有更大的抑制作用。这项研究为具有不同DP的PA的生物活性和微生物机制提供了新的见解。
