Abstract：
Patients diagnosed with T1a cancer undergo partial nephrectomy to remove the tumors. In the process of removing the tumors, loss of kidney volume is inevitable, and current surgical methods focus solely on hemostasis and wound closure. Here, we developed an implantable form of decellularized extracellular matrix sponge to target both hemostasis and wound healing at the lesion site. A porous form of kidney decellularized matrix was achieved by fabricating a chemically cross-linked cryogel followed by lyophilization. The prepared kidney decellularized extracellular matrix sponge (kdES) was then characterized for features relevant to a hemostasis as well as a biocompatible and degradable biomaterial. Finally, histological evaluations were made after implantation in rat kidney incision model. Both gelatin sponge and kdES displayed excellent hemocompatibility and biocompatibility. However, after a 4-week observation period, kdES exhibited more favorable wound healing results at the lesion site. This suggests a promising potential for kdES as a supportive material in facilitating wound closure during partial nephrectomy surgery. KdES not only achieved rapid hemostasis for managing renal hemorrhage that is comparable to commercial hemostatic sponges, but also demonstrated superior wound healing outcomes.
摘要：
诊断为T1a癌的患者接受部分肾切除术以切除肿瘤。在切除肿瘤的过程中，肾脏体积的损失是不可避免的，目前的手术方法仅集中在止血和伤口闭合上。这里,我们开发了一种可植入形式的脱细胞细胞外基质海绵，以靶向病变部位的止血和伤口愈合。多孔形式的肾去细胞化基质通过制造化学交联的冷冻凝胶然后冻干来实现。然后表征所制备的肾脏脱细胞细胞外基质海绵(kdES)的与止血以及生物相容性和可降解生物材料相关的特征。最后,在大鼠肾切口模型中植入后进行组织学评估。明胶海绵和kdES均表现出优异的血液相容性和生物相容性。然而,经过4周的观察期，kdES在病变部位表现出更有利的伤口愈合结果。这表明kdES作为支持材料在肾部分切除术期间促进伤口闭合的潜力很有希望。KdES不仅实现了与商业止血海绵相当的治疗肾出血的快速止血，但也表现出优越的伤口愈合效果。
