Abstract：
The mammary gland undergoes significant physiological changes as it undergoes a transition from virgin to pregnancy, lactation, and involution. However, the dynamic role of proteins in regulating these processes during mouse mammary gland development has not been thoroughly explored. In this study, we collected mouse mammary gland tissues from mature virgins aged 8-10 weeks (V), day 16 of pregnancy (P16d), day 12 of lactation (L12d), day 1 of forced weaning (FW 1d), and day 3 of forced weaning (FW 3d) stages for analysis using DIA-based quantitative proteomics technology. A total of 3,312 proteins were identified, of which 843 were DAPs that were categorized into nine clusters based on their abundance changes across developmental stages. Notably, DAPs in cluster 2, which peaked at the L12d stage, were primarily associated with mammary gland development and lactation. The protein-protein interaction network revealed that the epidermal growth factor (EGF) was central to this cluster. Our study provides a comprehensive overview of the mouse mammary gland development proteome and identifies some important proteins, such as EGF, Janus kinase 1 (JAK1), and signal transducer and activator of transcription 6 (STAT6) that may serve as potential targets for future research to provide guidelines for a deeper understanding of the developmental biology of mammary glands.
摘要：
乳腺经历了从处女到怀孕的转变，经历了显著的生理变化，哺乳期,和内卷。然而,在小鼠乳腺发育过程中，蛋白质在调节这些过程中的动态作用尚未得到彻底探索。在这项研究中,我们收集8-10周龄成熟处女的小鼠乳腺组织（V），怀孕第16天（P16d），哺乳期第12天（L12d），强制断奶的第1天（FW1d），和强制断奶（FW3d）阶段的第3天，使用基于DIA的定量蛋白质组学技术进行分析。总共鉴定了3,312种蛋白质，其中843是DAP，根据其在发育阶段的丰度变化分为9个簇。值得注意的是,集群2中的DAP，在L12d阶段达到峰值，主要与乳腺发育和泌乳有关。蛋白质-蛋白质相互作用网络表明，表皮生长因子（EGF）是该簇的核心。我们的研究提供了小鼠乳腺发育蛋白质组的全面概述，并确定了一些重要的蛋白质，比如EGF,Janus激酶1(JAK1),和信号转导和转录激活因子6（STAT6），可能作为未来研究的潜在目标，为更深入地了解乳腺发育生物学提供指导。
