Abstract：
Despite being a cytoplasmic protein abundant in neurons, tau is detectable in various extracellular fluids. In addition to being passively released from dying/degenerating neurons, tau is also actively released from living neurons in a neuronal activity-dependent mechanism. In vivo, tau released from neurons first appears in brain interstitial fluid (ISF) and subsequently drains into cerebrospinal fluid (CSF) by glymphatic system. Changes in CSF tau levels alter during the course of AD pathogenesis and are considered to predict the disease-progression of AD. A method to collect CSF from various mouse models of AD will serve as a valuable tool to investigate the dynamics of physiological/pathological tau released from neurons. In this chapter, we describe and characterize a method that reliably collects a relatively large volume of CSF from anesthetized mice.
摘要：
尽管是神经元中丰富的细胞质蛋白，tau可在各种细胞外液中检测到。除了被动地从死亡/退化的神经元中释放出来，tau也以神经元活动依赖性机制从活神经元中主动释放。在体内,从神经元释放的tau首先出现在脑间质液（ISF）中，随后通过淋巴系统排入脑脊液（CSF）。CSFtau水平的变化在AD发病过程中发生变化，被认为可以预测AD的疾病进展。从各种AD小鼠模型收集CSF的方法将作为研究从神经元释放的生理/病理tau的动力学的有价值的工具。在这一章中,我们描述并表征了一种可靠地从麻醉小鼠中收集相对大量CSF的方法。
