Abstract：
Salinity is a pivotal abiotic stress factor with far-reaching consequences on global crop growth, yield, and quality and which includes strawberries. R2R3-MYB transcription factors encompass a range of roles in plant development and responses to abiotic stress. In this study, we identified that strawberry transcription factor FaMYB63 exhibited a significant upregulation in its expression under salt stress conditions. An analysis using yeast assay demonstrated that FaMYB63 exhibited the ability to activate transcriptional activity. Compared with those in the wild-type (WT) plants, the seed germination rate, root length, contents of chlorophyll and proline, and antioxidant activities (SOD, CAT, and POD) were significantly higher in FaMYB63-overexpressing Arabidopsis plants exposed to salt stress. Conversely, the levels of malondialdehyde (MDA) were considerably lower. Additionally, the FaMYB63-overexpressed Arabidopsis plants displayed a substantially improved capacity to scavenge active oxygen. Furthermore, the activation of stress-related genes by FaMYB63 bolstered the tolerance of transgenic Arabidopsis to salt stress. It was also established that FaMYB63 binds directly to the promoter of the salt overly sensitive gene SOS1, thereby activating its expression. These findings identified FaMYB63 as a possible and important regulator of salt stress tolerance in strawberries.
摘要：
盐度是一个重要的非生物胁迫因子，对全球作物生长具有深远的影响。产量，和质量，其中包括草莓。R2R3-MYB转录因子在植物发育和对非生物胁迫的响应中包含一系列作用。在这项研究中,我们发现，在盐胁迫条件下，草莓转录因子FaMYB63的表达显着上调。使用酵母测定的分析表明FaMYB63表现出激活转录活性的能力。与野生型（WT）植物相比，种子发芽率，根长,叶绿素和脯氨酸的含量，和抗氧化活性(SOD，CAT,和POD）在暴露于盐胁迫的FaMYB63过表达的拟南芥植物中明显更高。相反，丙二醛（MDA）水平明显降低。此外,FaMYB63过表达的拟南芥植物表现出显著提高的清除活性氧的能力。此外，FaMYB63对胁迫相关基因的激活增强了转基因拟南芥对盐胁迫的耐受性。还确定FaMYB63直接结合盐过度敏感基因SOS1的启动子,从而激活其表达。这些发现确定FaMYB63是草莓耐盐胁迫的可能且重要的调节剂。
