Abstract：
The oriental fruit fly,Bactrocera dorsalis (Hendel), is a notorious pest of fruit crops, causing severe damage to fleshy fruits during oviposition and larval feeding. Gravid females locate suitable oviposition sites by detecting the host volatiles. Here, the oviposition preference of antenna-removed females and the electrophysiological response of ovipositors to benzothiazole indicated that both antennae and ovipositors are involved in perceiving benzothiazole. Subsequently, odorant receptors (ORs) expressed in both antennae and ovipositors were screened, and BdorOR43a-1 was further identified to respond to benzothiazole using voltage-clamp recording. Furthermore, BdorOR43a-1-/- mutants were obtained using the CRISPR/Cas9 system and their oviposition preference to benzothiazole was found to be significantly altered compared to WT females, suggesting that BdorOR43a-1 is one of the important ORs for benzothiazole perception. Our results not only demonstrate the important role of antennae and ovipositors in benzothiazole-induced oviposition but also elucidate on the OR responsible for benzothiazole perception in B. dorsalis.
摘要：
东方果蝇，dorsalis（Hendel），是一种臭名昭著的水果作物害虫，在产卵和幼虫喂养过程中对肉质水果造成严重损害。重力雌性通过检测宿主挥发物来定位合适的产卵位点。这里,摘除天线的雌性的产卵偏好和产卵器对苯并噻唑的电生理反应表明，天线和产卵器都参与感知苯并噻唑。随后，筛选了在触角和产卵器中表达的气味受体（ORs），使用电压钳记录进一步确定BdorOR43a-1对苯并噻唑有反应。此外，使用CRISPR/Cas9系统获得BdorOR43a-1-/-突变体，发现与WT雌性相比，它们对苯并噻唑的产卵偏好发生了显着变化，表明BdorOR43a-1是苯并噻唑感知的重要OR之一。我们的结果不仅证明了触角和产卵剂在苯并噻唑诱导的产卵中的重要作用，而且还阐明了在背芽孢杆菌中负责苯并噻唑感知的OR。
