Abstract：
UNASSIGNED: To analyze the significance of serum miR-145 and miR-210 expression levels in the diagnosis of carotid artery stenosis.
UNASSIGNED: During the same period, 55 healthy individuals who received physical examination in the same hospital were recruited as controls and assigned to a non-stenosis group. Among the included patients, there were 45 cases of mild stenosis, 14 cases of moderate stenosis, and 6 cases of severe stenosis after carotid color Doppler ultrasonography. The expression levels of miR-145 and miR-210 in serum were measured using real-time fluorescence quantitative polymerase chain reaction (qPCR) technology.
UNASSIGNED: The expression levels of serum miR-145 and miR-210 in carotid artery stenosis group were significantly lower than those in non-stenosis group (p < 0.001). Multivariate Logistic regression analysis showed that smoking history, diabetes, hypertension and total cholesterol were positively correlated with the occurrence of carotid artery stenosis (p < 0.05). The expression levels of miR-145 and miR-210 were significantly negatively correlated with carotid artery stenosis (p < 0.001). In addition, patients with carotid artery stenosis and low expression levels of miR-145 and miR-210 had a greater risk of cerebral ischemia (p < 0.05). Cox regression analysis showed that the low expression of miR-145 and miR-210 were independent predictors of cerebral ischemic events. ROC analysis confirmed that miR-145 and miR-210 had good diagnostic efficacy in cerebral ischemia (p < 0.001).
UNASSIGNED: The decreased expression of miR-145 and miR-210 in serum is closely related to the diagnostic significance of carotid artery stenosis, and may be used to predict the occurrence of cerebral ischemic events.
UNASSIGNED: During the same period, 55 healthy individuals who received physical examination in the same hospital were recruited as controls and assigned to a non-stenosis group. Among the included patients, there were 45 cases of mild stenosis, 14 cases of moderate stenosis, and 6 cases of severe stenosis after carotid color Doppler ultrasonography. The expression levels of miR-145 and miR-210 in serum were measured using real-time fluorescence quantitative polymerase chain reaction (qPCR) technology.
UNASSIGNED: The expression levels of serum miR-145 and miR-210 in carotid artery stenosis group were significantly lower than those in non-stenosis group (p < 0.001). Multivariate Logistic regression analysis showed that smoking history, diabetes, hypertension and total cholesterol were positively correlated with the occurrence of carotid artery stenosis (p < 0.05). The expression levels of miR-145 and miR-210 were significantly negatively correlated with carotid artery stenosis (p < 0.001). In addition, patients with carotid artery stenosis and low expression levels of miR-145 and miR-210 had a greater risk of cerebral ischemia (p < 0.05). Cox regression analysis showed that the low expression of miR-145 and miR-210 were independent predictors of cerebral ischemic events. ROC analysis confirmed that miR-145 and miR-210 had good diagnostic efficacy in cerebral ischemia (p < 0.001).
UNASSIGNED: The decreased expression of miR-145 and miR-210 in serum is closely related to the diagnostic significance of carotid artery stenosis, and may be used to predict the occurrence of cerebral ischemic events.
摘要：
■分析血清miR-145和miR-210表达水平在颈动脉狭窄诊断中的意义。
■在同一时期，在同一家医院接受体检的55名健康个体被招募为对照，并被分配到非狭窄组。在纳入的患者中，45例轻度狭窄,14例中度狭窄，6例重度狭窄患者行颈动脉彩色多普勒超声检查。采用实时荧光定量聚合酶链反应(qPCR)技术检测血清中miR-145和miR-210的表达水平。
■颈动脉狭窄组血清miR-145和miR-210表达水平显著低于非狭窄组(p<0.001)。多因素Logistic回归分析显示,吸烟史,糖尿病,高血压、总胆固醇与颈动脉狭窄的发生呈正相关(p<0.05)。miR-145和miR-210的表达水平与颈动脉狭窄呈显著负相关(p<0.001)。此外,颈动脉狭窄且miR-145和miR-210低表达的患者发生脑缺血的风险更大(p<0.05)。Cox回归分析显示miR-145和miR-210的低表达是脑缺血事件的独立预测因子。ROC分析证实miR-145和miR-210在脑缺血中具有良好的诊断功效(p<0.001)。
■血清中miR-145和miR-210的表达降低与颈动脉狭窄的诊断意义密切相关。并可用于预测脑缺血事件的发生。
■在同一时期，在同一家医院接受体检的55名健康个体被招募为对照，并被分配到非狭窄组。在纳入的患者中，45例轻度狭窄,14例中度狭窄，6例重度狭窄患者行颈动脉彩色多普勒超声检查。采用实时荧光定量聚合酶链反应(qPCR)技术检测血清中miR-145和miR-210的表达水平。
■颈动脉狭窄组血清miR-145和miR-210表达水平显著低于非狭窄组(p<0.001)。多因素Logistic回归分析显示,吸烟史,糖尿病,高血压、总胆固醇与颈动脉狭窄的发生呈正相关(p<0.05)。miR-145和miR-210的表达水平与颈动脉狭窄呈显著负相关(p<0.001)。此外,颈动脉狭窄且miR-145和miR-210低表达的患者发生脑缺血的风险更大(p<0.05)。Cox回归分析显示miR-145和miR-210的低表达是脑缺血事件的独立预测因子。ROC分析证实miR-145和miR-210在脑缺血中具有良好的诊断功效(p<0.001)。
■血清中miR-145和miR-210的表达降低与颈动脉狭窄的诊断意义密切相关。并可用于预测脑缺血事件的发生。
