PDF(Pubmed)
Abstract：
Inoculation with plant growth-promoting rhizobacteria (PGPR) strains has promoted plant growth and decreased nitrous oxide (N₂O) emissions from agricultural soils simultaneously. However, limited PGPR strains can mitigate N₂O emissions from agricultural soils, and the microbial ecological mechanisms underlying N₂O mitigation after inoculation are poorly understood. In greenhouse pot experiments, the effects of inoculation with Stutzerimonas stutzeri NRCB010 and NRCB025 on tomato growth and N₂O emissions were investigated in two vegetable agricultural soils with contrasting textures. Inoculation with NRCB010 and NRCB025 significantly promoted tomato growth in both soils. Moreover, inoculation with NRCB010 decreased the N₂O emissions from the fine- and coarse-textured soils by 38.7% and 52.2%, respectively, and inoculation with NRCB025 decreased the N₂O emissions from the coarse-textured soil by 76.6%. Inoculation with NRCB010 and NRCB025 decreased N₂O emissions mainly by altering soil microbial community composition and the abundance of nitrogen-cycle functional genes. The N₂O-mitigating effect might be partially explained by a decrease in the (amoA + amoB)/(nosZI + nosZII) and (nirS + nirK)/(nosZI + nosZII) ratios, respectively. Soil pH and organic matter were key variables that explain the variation in abundance of N-cycle functional genes and subsequent N₂O emission. Moreover, the N₂O-mitigating effect varied depending on soil textures and individual strain after inoculation. This study provides insights into developing biofertilizers with plant growth-promoting and N₂O-mitigating effects.
OBJECTIVE: Plant growth-promoting rhizobacteria (PGPR) have been applied to mitigate nitrous oxide (N₂O) emissions from agricultural soils, but the microbial ecological mechanisms underlying N₂O mitigation are poorly understood. That is why only limited PGPR strains can mitigate N₂O emissions from agricultural soils. Therefore, it is of substantial significance to reveal soil ecological mechanisms of PGPR strains to achieve efficient and reliable N₂O-mitigating effect after inoculation. Inoculation with Stutzerimonas stutzeri strains decreased N₂O emissions from two soils with contrasting textures probably by altering soil microbial community composition and gene abundance involved in nitrification and denitrification. Our findings provide detailed insight into soil ecological mechanisms of PGPR strains to mitigate N₂O emissions from vegetable agricultural soils.
OBJECTIVE: Plant growth-promoting rhizobacteria (PGPR) have been applied to mitigate nitrous oxide (N₂O) emissions from agricultural soils, but the microbial ecological mechanisms underlying N₂O mitigation are poorly understood. That is why only limited PGPR strains can mitigate N₂O emissions from agricultural soils. Therefore, it is of substantial significance to reveal soil ecological mechanisms of PGPR strains to achieve efficient and reliable N₂O-mitigating effect after inoculation. Inoculation with Stutzerimonas stutzeri strains decreased N₂O emissions from two soils with contrasting textures probably by altering soil microbial community composition and gene abundance involved in nitrification and denitrification. Our findings provide detailed insight into soil ecological mechanisms of PGPR strains to mitigate N₂O emissions from vegetable agricultural soils.
摘要：
接种促进植物生长的根际细菌（PGPR）菌株可促进植物生长，同时减少农业土壤中的一氧化二氮（N2O）排放。然而,有限的PGPR菌株可以减少农业土壤中的N2O排放，接种后缓解氮氧化物的微生物生态机制知之甚少。在温室盆栽实验中，在两种具有不同质地的蔬菜农业土壤中，研究了用StutzerionasNRCB010和NRCB025接种对番茄生长和氮排放的影响。接种NRCB010和NRCB025均能显著促进番茄生长。此外，接种NRCB010使细纹和粗纹土壤的氮氧化物排放量减少了38.7%和52.2%，分别,接种NRCB025可使粗化土壤的氮氧化物排放量减少76.6%。接种NRCB010和NRCB025主要通过改变土壤微生物群落组成和氮循环功能基因的丰度来减少氮排放。氮O缓解效应可能部分解释为(amoA+amoB)/(nosZI+nosZII)和(nirS+nirK)/(nosZI+nosZII)比率的降低，分别。土壤pH和有机质是解释N循环功能基因丰度变化和随后的NO2O排放的关键变量。此外，接种后，氮O缓解效果因土壤质地和单个菌株而异。这项研究提供了开发具有植物生长促进和氮O缓解作用的生物肥料的见解。
目的：植物生长促进根际细菌（PGPR）已用于减轻农业土壤中的一氧化二氮（N2O）排放，但对N2O缓解背后的微生物生态机制知之甚少。这就是为什么只有有限的PGPR菌株可以减少农业土壤中的N2O排放。因此,揭示PGPR菌株的土壤生态机制对接种后获得有效可靠的氮O缓解作用具有重要意义。接种Stutzerimonasstutzeri菌株可能通过改变土壤微生物群落组成和参与硝化和反硝化的基因丰度，减少了两种质地不同的土壤的NO2O排放。我们的发现为PGPR菌株减少蔬菜农业土壤中N2O排放的土壤生态机制提供了详细的见解。
目的：植物生长促进根际细菌（PGPR）已用于减轻农业土壤中的一氧化二氮（N2O）排放，但对N2O缓解背后的微生物生态机制知之甚少。这就是为什么只有有限的PGPR菌株可以减少农业土壤中的N2O排放。因此,揭示PGPR菌株的土壤生态机制对接种后获得有效可靠的氮O缓解作用具有重要意义。接种Stutzerimonasstutzeri菌株可能通过改变土壤微生物群落组成和参与硝化和反硝化的基因丰度，减少了两种质地不同的土壤的NO2O排放。我们的发现为PGPR菌株减少蔬菜农业土壤中N2O排放的土壤生态机制提供了详细的见解。
