Abstract：
BACKGROUND: Pine wood nematode (PWN; Bursaphelenchus xylophilus) is the causative agent of pine wilt disease (PWD), which is considered the most dangerous biohazard to conifer trees globally. The transmission of PWN relies on insect vectors, particularly the Japanese pine sawyer (JPS; Monochamus alternatus). However, the molecular mechanism underlying PWN-JPS assembly remains largely unknown.
RESULTS: Here, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full-length reference transcriptome for analyzing next-generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN-carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non-PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA-Seq, and found that fatty acid β-oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β-oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β-oxidation rate and increased PCA of JPS.
CONCLUSIONS: In conclusion, MaCPT1 was able to decrease the PWN-JPS assembly formation through the fatty acid β-oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS. © 2024 Society of Chemical Industry.
RESULTS: Here, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full-length reference transcriptome for analyzing next-generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN-carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non-PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA-Seq, and found that fatty acid β-oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β-oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β-oxidation rate and increased PCA of JPS.
CONCLUSIONS: In conclusion, MaCPT1 was able to decrease the PWN-JPS assembly formation through the fatty acid β-oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS. © 2024 Society of Chemical Industry.
摘要：
背景：松材线虫（PWN；松材线虫）是松材枯萎病（PWD）的病原体，这被认为是全球针叶树最危险的生物危害。PWN的传播依赖于昆虫媒介，特别是日本松树锯片（JPS；Monochamusalternatus）。然而,PWN-JPS组装的分子机制在很大程度上仍然未知。
结果：这里，我们发现，地域和性别都会显著影响JPS的PCA(PWN携带量)；因此，关于PWN负荷，探索了来自不同位置和性别的JPS转录组。由于基因组的短缺,我们开发了用于分析下一代测序数据的全长参考转录组.进行了比较基因组研究，通过使用报告的PWN和非PWN携带者昆虫物种的基因组，在JPS中提名了11248个潜在的携带PWN的相关基因（β）。然后，151个差异表达转录本（DES），其中28个与β重叠，与JPS的PCA相关的是由RNA-Seq提名的，发现脂肪酸β-氧化可能是影响JPSPCA的关键因素。此外，使用抑制剂Etomoxir实验降低了JPS脂肪酸β-氧化速率，导致JPS的PCA增加。同时,通过RNA干扰沉默JPS中的MaCPT1导致脂肪酸β-氧化速率降低和JPS的PCA增加。
结论：结论：MaCPT1能够通过JPS的脂肪酸β-氧化减少PWN-JPS组装体的形成。这些结果为探索PWN入侵对JPS的影响提供了新的见解。©2024化学工业学会。
结果：这里，我们发现，地域和性别都会显著影响JPS的PCA(PWN携带量)；因此，关于PWN负荷，探索了来自不同位置和性别的JPS转录组。由于基因组的短缺,我们开发了用于分析下一代测序数据的全长参考转录组.进行了比较基因组研究，通过使用报告的PWN和非PWN携带者昆虫物种的基因组，在JPS中提名了11248个潜在的携带PWN的相关基因（β）。然后，151个差异表达转录本（DES），其中28个与β重叠，与JPS的PCA相关的是由RNA-Seq提名的，发现脂肪酸β-氧化可能是影响JPSPCA的关键因素。此外，使用抑制剂Etomoxir实验降低了JPS脂肪酸β-氧化速率，导致JPS的PCA增加。同时,通过RNA干扰沉默JPS中的MaCPT1导致脂肪酸β-氧化速率降低和JPS的PCA增加。
结论：结论：MaCPT1能够通过JPS的脂肪酸β-氧化减少PWN-JPS组装体的形成。这些结果为探索PWN入侵对JPS的影响提供了新的见解。©2024化学工业学会。
