RESULTS: Here, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full-length reference transcriptome for analyzing next-generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN-carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non-PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA-Seq, and found that fatty acid β-oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β-oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β-oxidation rate and increased PCA of JPS.
CONCLUSIONS: In conclusion, MaCPT1 was able to decrease the PWN-JPS assembly formation through the fatty acid β-oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS. © 2024 Society of Chemical Industry.
结果:这里,我们发现,地域和性别都会显著影响JPS的PCA(PWN携带量);因此,关于PWN负荷,探索了来自不同位置和性别的JPS转录组。由于基因组的短缺,我们开发了用于分析下一代测序数据的全长参考转录组.进行了比较基因组研究,通过使用报告的PWN和非PWN携带者昆虫物种的基因组,在JPS中提名了11248个潜在的携带PWN的相关基因(β)。然后,151个差异表达转录本(DES),其中28个与β重叠,与JPS的PCA相关的是由RNA-Seq提名的,发现脂肪酸β-氧化可能是影响JPSPCA的关键因素。此外,使用抑制剂Etomoxir实验降低了JPS脂肪酸β-氧化速率,导致JPS的PCA增加。同时,通过RNA干扰沉默JPS中的MaCPT1导致脂肪酸β-氧化速率降低和JPS的PCA增加。
结论:结论:MaCPT1能够通过JPS的脂肪酸β-氧化减少PWN-JPS组装体的形成。这些结果为探索PWN入侵对JPS的影响提供了新的见解。©2024化学工业学会。