PDF(Pubmed)
Abstract：
Streptococcus suis is a bacterial gram-positive pathogen that causes invasive infections in swine and is also a zoonotic disease agent. Traditional molecular typing techniques such as ribotyping, multilocus sequence typing, pulse-field gel electrophoresis, or randomly amplified polymorphic DNA have been used to investigate S. suis population structure, evolution, and genetic relationships and support epidemiological and virulence investigations. However, these traditional typing techniques do not fully reveal the genetically heterogeneous nature of S. suis strains. The high-resolution provided by whole-genome sequencing (WGS), which is now more affordable and more commonly available in research and clinical settings, has unlocked the exploration of S. suis genetics at full resolution, permitting the determination of population structure, genetic diversity, identification of virulent clades, genetic markers, and other bacterial features of interest. This approach will likely become the new gold standard for S. suis strain typing as WGS instruments become more widely available and traditional typing techniques are gradually replaced.
摘要：
猪链球菌是一种细菌革兰氏阳性病原体，可引起猪的侵袭性感染，也是人畜共患疾病的病原体。传统的分子分型技术，如核糖核酸分型，多位点序列分型,脉冲场凝胶电泳,或随机扩增的多态性DNA已用于研究猪链球菌种群结构，进化,和遗传关系，并支持流行病学和毒力调查。然而,这些传统的分型技术不能完全揭示猪链球菌菌株的遗传异质性。全基因组测序（WGS）提供的高分辨率，现在在研究和临床环境中更便宜，更常见，已经完全解锁了对猪链球菌遗传学的探索，允许确定人口结构，遗传多样性,毒力进化枝的鉴定，遗传标记,和其他感兴趣的细菌特征。随着WGS仪器变得越来越广泛并且传统的分型技术逐渐被取代，这种方法可能会成为猪链球菌菌株分型的新黄金标准。
