Abstract：
Salvia miltiorrhiza, a widely used medicinal herb renowned for its properties in promoting blood circulation, removing blood stasis and alleviating pain, is currently facing quality degradation due to excessive heavy metal levels, posing a threat to medication safety. In order to investigate the effects of microbial inoculant, microalgae and biochar on the growth of Salvia miltiorrhiza under copper (Cu) stress, as well as its Cu absorption, antioxidant activity, active component contents and rhizosphere microbial community, a pot experiment was conducted. Salvia miltiorrhiza plants were cultivated in the soil containing 400 mg/kg of Cu for six months and treated with microbial inoculant, microalgae and biochar, either individually or in combination. Almost all soil amendment treatments led to an increase in root biomass. Notably, co-application of microbial inoculant and microalgae had the optimal effect with a 63.07 % increase compared to the group treated solely with Cu. Moreover, when microbial inoculant was applied alone or in combination with microalgae, the Cu content in plant roots was reduced by 19.29 % and 25.37 %, respectively, whereas other treatments failed to show a decreasing trend. Intriguingly, Cu stress increased the active component contents in plant roots, and they could also be enhanced beyond non-stress levels when microbial inoculant and microalgae were applied together or in combination with biochar. Analyses of plant antioxidant activity, soil properties and rhizosphere microorganisms indicated that these amendments may alleviate Cu stress by enhancing peroxidase activity, facilitating plant nutrient absorption, and enriching beneficial microorganisms capable of promoting plant growth and mitigating heavy metal-induced damage. This study suggests that the combined application of microbial inoculant and microalgae can reduce Cu levels in Salvia miltiorrhiza while enhancing its quality under Cu stress.
摘要：
丹参,一种广泛使用的草药，以其促进血液循环的特性而闻名，祛瘀止痛,目前由于重金属含量过高而面临质量下降，对用药安全构成威胁。为了研究微生物接种剂的效果，微藻和生物炭对铜(Cu)胁迫下丹参生长的影响,以及它的铜吸收，抗氧化活性，活性成分含量和根际微生物群落，进行了盆栽实验。将丹参植物在含400mg/kgCu的土壤中种植六个月，并用微生物菌剂处理，微藻和生物炭，无论是单独或组合。几乎所有土壤改良处理都导致根生物量增加。值得注意的是,与仅用Cu处理的组相比,微生物接种剂和微藻的共同施用具有63.07%的最佳效果。此外，当微生物接种剂单独或与微藻联合使用时，植物根中的Cu含量分别降低了19.29%和25.37%,分别,而其他治疗未能显示出下降趋势。有趣的是，Cu胁迫增加了植物根系中活性成分的含量,当微生物接种剂和微藻一起使用或与生物炭结合使用时，它们也可以增强到非胁迫水平以上。植物抗氧化活性分析,土壤性质和根际微生物表明，这些改良剂可能通过增强过氧化物酶活性来缓解铜胁迫，促进植物营养吸收，和富集能够促进植物生长和减轻重金属诱导的损害的有益微生物。该研究表明，微生物接种剂和微藻的联合应用可以降低丹参中的Cu含量，同时提高其在Cu胁迫下的质量。
