Abstract：
A novel bifunctional MOF-encapsulated cobalt-doped carbon dots nanozyme (Co-CD/PMOF) with excellent peroxidase-mimic catalytic activity and fluorescence property was synthesized and employed to fabricate a chemiluminescence/fluorescence (CL/FL) dual-mode immunosensor for AFB1 detection. Co-CD/PMOF could catalyze the luminol/H2O2 system to generate robust and long-lasting CL signals due to the slow diffusion effect and continuous generation of •OH, O2•-, and 1O2 species. Differing from traditional flash-type CL emissions, this glow-type CL emission is helpful to fabricate a sensitive and accurate CL sensing platform. Then the CL/FL dual-mode detection of AFB1 was developed using antibody-functionalized Co-CD/PMOF as the signal-amplifying nanoprobe. The CL mode assay based on indirect competitive immune principle was carried out on a chemiluminescence optical fiber platform, where the AFB1-OVA-functionalized optical fiber probe was employed for biorecognition, separation, and signal conducting. The AFB1 detection range and LOD were 0.63-69.36 ng/mL and 0.217 ng/mL, respectively. Using AFB1 antibody-functionalized immunomagnetic beads for capturing and separation, the FL mode detection of AFB1 was established based on the sandwich immune principle. A linear range of 0.54-51.91 ng/mL and a LOD of 0.027 ng/mL were obtained. This work designed a sensitive, rapid, and reliable nanozyme-powered dual-mode assay strategy and provided technical support in the field of environmental monitoring and food safety.
摘要：
合成了一种具有优异的过氧化物酶模拟催化活性和荧光特性的新型双功能MOF包裹的钴掺杂碳点纳米酶（Co-CD/PMOF），并将其用于化学发光/荧光（CL/FL）双模式免疫传感器用于AFB1检测。Co-CD/PMOF可以催化鲁米诺/H2O2系统产生强大和持久的CL信号，由于缓慢的扩散效应和•OH的连续产生，O2•-，和1O2物种。不同于传统的闪光型CL排放，这种辉光型CL发射有助于制造灵敏和精确的CL传感平台。然后使用抗体官能化的Co-CD/PMOF作为信号放大纳米探针开发AFB1的CL/FL双模式检测。在化学发光光纤平台上进行了基于间接竞争免疫原理的CL模式测定，其中AFB1-OVA功能化的光纤探针用于生物识别，分离,和信号传导。AFB1检测范围和LOD分别为0.63-69.36ng/mL和0.217ng/mL，分别。使用AFB1抗体功能化的免疫磁珠进行捕获和分离，基于夹心免疫原理建立AFB1的FL模式检测。得到0.54-51.91ng/mL的线性范围和0.027ng/mL的LOD。这项工作设计了一个敏感的，快速,和可靠的纳米酶动力双模式检测策略，并在环境监测和食品安全领域提供技术支持。
