Abstract：
Switching from ELISpot to FluoroSpot enables the analysis of spot-forming units representing cells producing different cytokines as well as the frequencies of spots derived from cells co-secreting multiple cytokines. Due to the fluorescent read-out signal, sophisticated reader instruments can also measure the relative spot volume, making it possible to differentiate between spots generated by cells secreting different levels of one or more cytokines. Here we describe how triple FluoroSpot assays can be used to define polyfunctional T cells secreting multiple cytokines and how different T-cell populations can differ in the levels of cytokines they secrete.
摘要：
从ELISpot切换到FluoroSpot使得能够分析代表产生不同细胞因子的细胞的斑点形成单位以及源自共分泌多种细胞因子的细胞的斑点的频率。由于荧光读出信号,先进的阅读器仪器还可以测量相对光斑体积，使得可以区分分泌不同水平的一种或多种细胞因子的细胞产生的斑点。在这里，我们描述了如何使用三重FluororoSpot测定来定义分泌多种细胞因子的多功能T细胞，以及不同的T细胞群体在它们分泌的细胞因子水平上如何不同。
