UNASSIGNED: This extensive review of the literature discusses the performance of Mucorales-specific PCR and other genus-specific or broad-range fungal PCR that can be used for the diagnosis of NAIMI in diverse clinical samples, with a focus on novel technologies.
UNASSIGNED: PCR currently represents the most promising approach, combining good sensitivity/specificity and ability to detect NAIMI in clinical samples before diagnosis by conventional cultures and histopathology. Several PCR assays have been designed for the detection of Mucorales in particular, but also Fusarium spp. or Scedosporium/Lomentospora spp. Some commercial Mucorales PCRs are now available. While efforts are still needed for standardized protocols and the development of more rapid and simpler techniques, PCR is on the way to becoming an essential test for the early diagnosis of mucormycosis and possibly other NAIMIs.
■这篇广泛的文献综述讨论了Mucorales特异性PCR和其他属特异性或广泛真菌PCR的性能,可用于诊断各种临床样品中的NAIMI,专注于新技术。
■PCR目前是最有前途的方法,结合良好的敏感性/特异性和在诊断前通过常规培养和组织病理学检测临床样本中NAIMI的能力。已经设计了几种PCR检测方法,特别是用于检测Mucorales,还有镰刀菌.或Scedosporium/Lomentosporaspp。一些商业MucoralesPCR现在可用。虽然标准化协议和开发更快速和更简单的技术仍然需要努力,PCR正在成为毛霉菌病和其他NAIMIs早期诊断的重要测试。