PDF(Pubmed)
Abstract：
Ovarian cancer (OC) is deadly, and likely arises from the fallopian tube epithelium (FTE). Despite the association of OC with ovulation, OC typically presents in post-menopausal women who are no longer ovulating. The goal of this study was to understand how ovulation and aging interact to impact OC progression from the FTE. Follicular fluid released during ovulation induces DNA damage in the FTE, however, the role of aging on FTE exposure to follicular fluid is unexplored. Follicular fluid samples were collected from 14 women and its effects on FTE cells was assessed. Follicular fluid caused DNA damage and lipid oxidation in an age-dependent manner, but instead induced cell proliferation in a dose-dependent manner, independent of age in FTE cells. Follicular fluid regardless of age disrupted FTE spheroid formation and stimulated attachment and growth on ultra-low attachment plates. Proteomics analysis of the adhesion proteins in the follicular fluid samples identified vitronectin, a glycoprotein responsible for FTE cell attachment and spreading.
摘要：
卵巢癌（OC）是致命的，并且可能来自输卵管上皮（FTE）。尽管OC与排卵有关，OC通常出现在不再排卵的绝经后妇女中。这项研究的目的是了解排卵和衰老如何相互作用以影响FTE的OC进展。排卵过程中释放的卵泡液诱导FTE中的DNA损伤，然而,衰老对FTE暴露于卵泡液的作用尚未探讨。从14名女性收集卵泡液样品并评估其对FTE细胞的影响。卵泡液以年龄依赖性方式引起DNA损伤和脂质氧化，而是以剂量依赖的方式诱导细胞增殖，与FTE细胞的年龄无关。无论年龄大小，卵泡液都会破坏FTE球体的形成，并刺激超低附着板上的附着和生长。卵泡液样品中粘附蛋白的蛋白质组学分析鉴定了玻连蛋白，一种负责FTE细胞附着和扩散的糖蛋白。
